原创力文档Autoprocessing of human immunodeficiency virus type 1 protease miniprecursor fusions in mammalian cells.docVIP
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Autoprocessing of human immunodeficiency virus type 1 protease miniprecursor fusions in mammalian cells
HuangandChenAIDSResearchandTherapy2010,7:27
/content/7/1/27
RESEARCH
OpenAccess
Autoprocessingofhumanimmunodeficiency
virustype1proteaseminiprecursorfusionsin
mammaliancells
LiangqunHuang,ChaopingChen*
Abstract
Background:HIVprotease(PR)isavirus-encodedasparticproteasethatisessentialforviralreplicationand
infectivity.ThefullyactiveandmaturedimericproteaseisreleasedfromtheGag-Polpolyproteinasaresultof
precursorautoprocessing.
Results:WeheredescribeasimplemodelsystemtodirectlyexamineHIVproteaseautoprocessingintransfected
mammaliancells.AfusionprecursorwasengineeredencodingGSTfusedtoawell-chara
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