Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs.docVIP

Frontiers in Zoology BioMedCentral Methodology Open Access Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs Hege Vestheim*1 and Simon N Jarman2 Address: 1Department of Biology, University of Oslo, P.O. Box 1066, Blindern, 0316, Oslo, Norway and 2Australian Antarctic Division, 203 Channel Highway, Kingston, TAS, 7050, Australia Email: Hege Vestheim* - h.f.vestheim@bio.uio.no; Simon N Jarman - simon.jarman@.au * Corresponding author Published: 20 July 2008 Received: 6 March 2008 Accepted: 20 July 2008