原创力文档Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I.docVIP
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Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I
CompetitiveRT-PCRStrategyforQuantitativeEvaluation
oftheExpressionofTilapia(Oreochromisniloticus )Growth
HormoneReceptorTypeI
AlinaRodríguez-Mallon,YamiletCárdenas,JuanaMaríaLugo,
AyméOliva,AntonioMorales,andMarioPabloEstrada
Abstract
Quantizationofgeneexpressionrequiresthatanaccuratemeasurementofaspecifictranscriptismade.Inthis
paper,aquantitativereversetranscription-polymerasechainreaction(RT-PCR)bycompetitionfortilapia
growthhormonereceptortypeIisdesignedandvalidated.Thisexperimentalprocedurewasusedtodetermine
theabundanceofgrowthhormonereceptortypeItranscriptindifferenttilapiatissues.The
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