Distribution and sources of variability of sister-chromatid exchange frequencies in cattle.docVIP
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Distribution and sources of variability of sister-chromatid exchange frequencies in cattle
J Catalán C Moreno MV Arruga
1
2
Quantitative Genetics Unit, Veterinary Faculty, Miguel Servet, 177,
50013-Zaragoza, Spain
(Received 25 August 1993; accepted 12 October 1993)
Summary - The spontaneous incidence of sister-chromatid exchange (SCE) was investi-
gated in a group of cattle, composed of 24 animals of both sexes belonging to different
breeds, ages and farms. Thework was carried out following a randomized block design. The
mean value of SCEs/cell was 5.77 :f:: 0.082 using 5 ltg/ml of bromodeoxyuridine (BrdU).
The distribution of SCE frequency fits the Poisson model fairly well, although the neg-
ative binomial model also gave a good representation of exchange distribution. Among
the analyzed sources of variability, group, animal and treatment of BrdU factors showed
significant effects.
of BrdU molecules available per cell has little influence on SCE rates in relation to its
molarity.
A
sister-chromatid exchange / distribution frequency / bromodeoxyuridine / cattle
Résumé -Distribution et sources de variation des fréquences d’échange entre chroma-
tides-soeurs chez les bovins. L’incidence spontanée des échanges entre chromatides-sceurs
a été étudiée chez 24 bovins de différents sexes, races, ages et eaploitations. L’étude
été menée suivant un dispositif en blocs randomisés. Le nombre moyen ECS/cellule
été de 5,77f 0,082 en utilisant 5 gg/ml de bromodéoxyuridine (BrdU). La distribution de
fréquence des
a
a
ECS suit
un modèle de Poisson, bien qu’un modèle binomial
principalement
négatif donne aussi une bonne représentation de la distribution. Parmi les sources de
variation analysées, les facteurs groupe, animal et traitement du BrdU ont montré des
effets significatifs. Un tmitement de BrdU introduit récemment a montré que le nombre
de molécules de BrdU disponibles par cellule a une légère influence sur le tau! d’ECS en
relation avec sa molarité.
échange entre chromatides-sceurs / distribution de fréquence / bromodéoxyuridine /
bo
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