Effect of Paeonol on Antioxidant and Immune Regulatory Activity in Hepatocellular Carcinoma Rats.docVIP

Molecules 2012, 17, 4672-4683; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Effect of Paeonol on Antioxidant and Immune Regulatory Activity in Hepatocellular Carcinoma Rats Bendong Chen 1,2, Mingliang Ning 3 and Guangshun Yang 1,* 1 Department of Fifth Hepatic Surgery, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438, China 2 Department of Hepatobiliary Surgery, The General Hospital of Ningxia Medical University, Yinchuan 750004, China 3 Department of Oncological Surgery, The General Hospital of Ningxia Medical University, Yinchuan 750004, China * Author to whom correspondence should be addressed; E-Mail: ghyangsjyd@; Tel./Fax: +86-21-8187-5291. Received: 8 March 2012; in revised form: 27 March 2012 / Accepted: 5 April 2012 / Published: 20 April 2012 Abstract: The study investigated the immunity and antioxidant potential of paeonol by employing a hepatocellular carcinoma (HCC) rat model. Three doses of paeonol (20, 40, 60 mg/kg b.w. orally) were administrated to diethylnitrosamine (DEN)-induced HCC rats. Results showed that paeonol significantly reduced the serum AST, ALT, ALP, GGT, AFU and liver MDA levels, increased serum WBC, TP, ALB, A/G, TNF-α and IFN-γ and liver antioxidant enzymes activities (SOD, CAT, GSH-Px, GR) in HCC rats. Altogether, these results suggest that the paeonol could effectively decrease oxidative injury and improve immunity function in HCC rats. Keywords: hepatocellular carcinoma; paeonol; oxidative injury; immunity; diethyl-nitrosamine 1. Introduction Hepatocellular carcinoma is the most common and lethal of all cancers. A diversity of dietary [1], endogenous and environmental [2] stimuli mediate hepatocarcinogenesis. Diethylnitrosamine is an N-nitroso alkyl compound, categorized as a potent hepatotoxin and hepatocarcinogen in experimental  Molecules 2012, 17 4673 animals, producing reproducible tumors a