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Effects of Lactobacillus rhamnosus GG supplementation on cows milk allergy in a mouse model.doc

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Effects of Lactobacillus rhamnosus GG supplementation on cows milk allergy in a mouse model

Thangetal.Allergy,AsthmaClinicalImmunology2011,7:20 /content/7/1/20 ALLERGY, ASTHMA CLINICAL IMMUNOLOGY RESEARCH OpenAccess EffectsofLactobacillusrhamnosusGG supplementationoncow’smilkallergyina mousemodel CinLThang1,BushansinghBaurhoo1,JoyceIBoye2,BenjaminKSimpson3andXinZhao1* Abstract Background:Cow’smilkallergy(CMA)isoneofthemostprevalenthumanfood-borneallergies,particularlyin infantsandyoungchildrenfromdevelopedcountries.OurstudyaimstoevaluatetheeffectsofLactobacillus rhamnosusGG(LGG)administrationonCMAdevelopmentusingwholecow’smilkproteins(CMP)sensitizedBalb/C micebytwodifferentsensitizationmethods. Methods:LGGsupplementedmicewereeithersensitizedorallywithCMPandcholeratoxinB-subunit(CTB)as adjuvant,orintraperitoneally(IP)withCMPbutwithouttheadjuvant.MicewerethenorallychallengedwithCMP andallergicresponseswereaccessedbymonitoringhypersensitivityscores,measuringthelevelsofCMP-specific immunoglobulins(IgG1,IgG2aandIgG)andtotalIgEfromsera,andcytokines(IL-4andIFN-g)fromspleenlysates. Results:SensitizationtoCMPwassuccessfulonlyinIPsensitizedmice,butnotinorallysensitizedmicewithCMP andCTB.Interestingly,LGGsupplementationappearedtohavereducedcow’smilkallergy(CMA)intheIPgroupof mice,asindicatedbyloweredallergicresponses. Conclusions:Adjuvant-freeIPsensitizationwithCMPwassuccessfulininducingCMAintheBalb/Cmicemodel. LGGsupplementationfavourablymodulatedimmunereactionsbyshiftingTh2-dominatedtrendstowardTh1- dominatedresponsesinCMPsensitizedmice.Ourresultsalsosuggestthatoralsensitizationbytheco- administrationofCMPandCTB,asadjuvant,mightnotbeappropriatetoinduceCMAinmice. Background approaches: toalter the structure and property ofmilk Cow’smilkallergy(CMA),animmunologicallymediated proteins through thermal treatments, biochemical pro- reaction to cow’s milk proteins [1], is one of the most cesses (enzymatic digestion), irradiation [3] and high prevalent human food-borne allergies, particularly in pressure treatments [4], and to modulate immune infantsandyoungchil