Effects of redox cycling compounds on DT diaphorase activity in the liver of rainbow trout (Oncorhynchus mykiss).docVIP

Comparative Hepatology BioMedCentral Research Open Access Effects of redox cycling compounds on DT diaphorase activity in the liver of rainbow trout (Oncorhynchus mykiss) Joachim Sturve*, Eiríkur Stephensen and Lars F?rlin Address: Department of Zoology, Zoophysiology, G?teborg University, Box 463, 405 30, G?teborg, Sweden Email: Joachim Sturve* - joachim.sturve@zool.gu.se; Eiríkur Stephensen - eirikur@lyf.is; Lars F?rlin - lars.forlin@zool.gu.se * Corresponding author Published: 04 May 2005 Received: 22 December 2004 Accepted: 04 May 2005 Comparative Hepatology 2005, 4:4 doi:10.1186/1476-5926-4-4 This article is available from: /content/4/1/4 ? 2005 Sturve et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: DT diaphorase (DTD; NAD(P)H:quinone oxidoreductase; EC ) catalyses the two electron reduction of quinones, thus preventing redox cycling and consequently quinone dependent production of reactive oxygen species. In rat and mouse, a wide range of chemicals including polyaromatic hydrocarbons, azo dyes and quinones induces DTD. Bifunctional compounds, such asβ-naphthoflavone (β-NF) and benzo(a)pyrene (B(a)P), induce DTD together with CYP1A and phase II enzymes by a mechanism involving the aryl hydrocarbon receptor (AHR). Monofunctional induction of DTD is mediated through the antioxidant response element and does not lead to the induction of AHR dependent enzymes, such as CYP1A. The aim of this study was to investigate the effects of prooxidants (both bifunctional and monofunctional) on the activity of hepatic DTD in rainbow trout (Oncorhynchus mykiss) in order to evaluate DTD suitability as a biomarker. We also investigated the effect of β-