Emodin-8-O-β-D-Glucoside from Polygonum Amplexicaule D. Don var. Sinense Forb. Promotes Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells.docVIP

下载本文档

10
0
约2.52万字
约 10页
2017-05-16 发布于上海
举报
版权申诉

Emodin-8-O-β-D-Glucoside from Polygonum Amplexicaule D. Don var. Sinense Forb. Promotes Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells.doc

1、本文档共10页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Emodin-8-O-β-D-Glucoside from Polygonum Amplexicaule D. Don var. Sinense Forb. Promotes Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells

Molecules 2011, 16, 728-737; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Emodin-8-O-β-D-Glucoside from Polygonum Amplexicaule D. Don var. Sinense Forb. Promotes Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells Mei-Xian Xiang 1,2, Zong Xu 2, Han-Wen Su 3, Jinyue Hu 3,* and Yun-Jun Yan 1,* 1 College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China 2 College of Pharmacy, South-Central University for Nationalities, Wuhan 430075, China 3 Renmin Hospital, Wuhan University, Wuhan 430060, China * Authors to whom correspondence should be addressed: E-Mails: yanyunjun@ (T-J.Y.); jinyuehu@ (J.H.); Tel.: +86-02712; Fax: +86-027 Received: 26 November 2010; in revised form: 7 January 2011 / Accepted: 17 January 2011 / Published: 18 January 2011 Abstract: Polygonum amplexicaule D. Don var. sinense Forb. (Polygonaceae) (PAF) is a famous traditional herb used to treat fractures, rheumatoid arthritis, muscle injury and pain. The present study was designed to investigate a PAF derived-chemical compound emodin- 8-O-β-D-glucoside (EG) on the proliferation and differentiation of osteoblastic MC3T3-E1 cell in vitro. A compound was isolated from PAF extract by HPLC and identified as emodin-8-O-β-D-glucoside (EG) by spectroscopic methods. EG significantly promoted cell proliferation at 0.1–100 ng/mL, and increased the cell proportion in S-phase from 16.34% to 32.16%. Moreover, EG increased alkaline phosphatase (ALP) expression in MC3T3-E1 cells at the concentration from 0.1 to 100 ng/mL and inhibited PGE2 production induced by TNF- α in osteoblasts at the concentrations ranging from 10–100 ng/mL, suggesting that cell differentiation was induced in MC3T3-E1 osteoblasts. Taken together, these results indicated compound EG directly stimulated cell proliferation and differentiation of osteobla