Evaluation of Protein Adsorption on Chitosan Surfaces with Reflectometry Interference Spectroscopy.docVIP

Sensors 2001, 1, 148-160 sensors ISSN 1424-8220 ? 2001 by MDPI /sensors Evaluation of Protein Adsorption on Chitosan Surfaces with Reflectometry Interference Spectroscopy Xiao Ying Lü?, Yan Huang and Chao Qun Ma Key Laboratory of Molecular and Biomolecular Electronics of the Ministry of Education, P. R. China. Southeast University, 210096, Nanjing, China. Tel: (86)-25-3793430. Fax: (86)-25-7712719. E-mail: luxy@. Homepage: * Author to whom correspondence should be addressed. Received: 4 September 2001 / Accepted: 25 September 2001 / Revised: 10 October 2001 / Published: 12 October 2001 Abstract: Using a biomedical sensor setup RIfS we have investigated the kinetic behavior of human albumin (Alb), human fibrinogen (Fib), and human immunoglobulin G (IgG) adsorbed onto surfaces of chitosan. Polystyrene (PS) was used as the control material in this study. The optical thickness of three kinds of proteins measured by RIfS was related to their molecular dimensions and potential orientations on a film surface. According to the operation principle of RIfS and the molecular dimensions of three kinds of proteins, the adsorbed layers of proteins onto the surface of chitosan and PS was calculated by using a newly introduced equation. The microstructure of the chitosan and polystyrene film and the surfaces with adsorbed proteins were imaged by atomic force microscopy (AFM). With AFM analyses the lateral distribution of the protein molecules on surfaces have been recognized. The results show that the number of adsorbed layers of the three proteins on the surface of chitosan are 0.635 for Alb, 0.158 for Fib and 0.0967 for IgG, and of polystyrene are: 0.577 for IgG, 0.399 for Fib, 0.336 for Alb. This study confirmed that RIfS is a useful tool for the analysis of plasma proteins adsorbed on a surface of biomaterials. Results show that at first on the surface of chitosan film much more Alb