Expression of inflammatory host genes in Chlamydia trachomatis-infected human monocytes.docVIP

Available online /content/9/3/R54 Research article Open Access Vol 9 No 3 Expression of inflammatory host genes in Chlamydia trachomatis-infected human monocytes Sina Schrader1, Andreas Klos2, Simone Hess3, Henning Zeidler1, Jens G Kuipers4 and Markus Rihl1 1Division of Rheumatology, Hannover Medical School (MHH), Carl-Neuberg-Str. 1, 30625 Hannover, Germany 2Department of Medical Microbiology and Hospital Epidemiology, Hannover Medical School (MHH), Carl-Neuberg-Str. 1, 30625 Hannover, Germany 3Department of Molecular Biology, Max Planck Institute for Infection Biology, 10117 Berlin, Germany 4Division of Rheumatology, Rotes Kreuz Krankenhaus, St.-Pauli-Deich 24, 29199 Bremen, Germany Corresponding author: Markus Rihl, rihl.markus@mh-hannover.de Received: 7 Nov 2006 Revisions requested: 4 Dec 2006 Revisions received: 22 Feb 2007 Accepted: 24 May 2007 Published: 24 May 2007 Arthritis Research Therapy 2007, 9:R54 (doi:10.1186/ar2209) This article is online at: /content/9/3/R54 ? 2007 Schrader et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract The aim of this study was to perform a comprehensive gene analysis of cytokines, chemokines, and their receptors were found to be upregulated exclusively in the early, active phase of the infection as compared to four genes in the late, persistent state of the infection. Apart from receptors, both the level and the number of transcripts encoding inflammatory products decreased with ongoing infection. Four genes (interferon-gamma, macrophage inflammatory protein [MIP]-1- alpha, MIP-1-beta, and interleukin-2 receptor-gamma) were constantly expressed over a period of 7 days. The current study provides data on the induction of mRNA encoding