Fast and Sensitive LC-DAD-ESIMS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu).docVIP

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Fast and Sensitive LC-DAD-ESIMS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu).doc

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Fast and Sensitive LC-DAD-ESIMS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu)

Molecules 2011, 16, 1533-1543; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu) Li-Chun Zhao 1,?, Wei Li 2,?, Yu-Fang He 3, Xiao-Hua Li 4, Zi Wang 2, Wen-Cong Liu 2, Yi-Nan Zheng 2 and Jian Liang 1,* 1 The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College, Nanning 530011, China; E-Mail: hyzlc@126.com (L.-C.Z.) 2 College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China 3 Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China 4 Department of Crop Science, Chungnam National University, Daejeon 305-754, Korea ? These two authors contributed equally to this work and should be considered as co-first author. * Authors to whom correspondence should be addressed; E-Mail: ljruikang@126.com; Tel.: +86-771-218-8589; Fax: +86-771-233-5098. Received: 27 December 2010; in revised form: 17 January 2011 / Accepted: 20 January 2011 / Published: 11 February 2011 Abstract: In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of sa