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Faster differentiation of Staphylococcus aureus versus coagulase-negative Staphylococci from blood culture material a comparison of different bacterial DNA isolation methods.doc

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Faster differentiation of Staphylococcus aureus versus coagulase-negative Staphylococci from blood culture material a comparison of different bacterial DNA isolation methods

Available online /supplements/13/S4 Critical Care Volume 13 Suppl 4, 2009 Sepsis 2009 Amsterdam, the Netherlands, 11–14 November 2009 Published online: 11 November 2009 These abstracts are available online at /supplements/13/S4 ? 2009 BioMed Central Ltd P1 P2 Urokinase receptor is necessary for bacterial defense against Gram-negative sepsis (melioidosis) by facilitating phagoctytosis A comparison of acute lung inflammation inKlebsiella pneumoniae B5055-induced pneumonia and sepsis in BALB/c mice W Joost Wiersinga1,2, JWR Hovius1,2, GJW van der Windt1,2, JCM Meijers3, JJ Roelofs4, A Dondorp5, M Levi1, NP Day5,6, SJ Peacock5,6, T van der Poll1,2 V Kumar, S Chibber Department of Microbiology, Panjab University, Chandigarh, India Critical Care 2009, 13(Suppl 4):P2 (doi: 10.1186/cc8058) 1Center for Infection and Immunity Amsterdam, 2Center for Experimental and Molecular Medicine, 3Department of Vascular Medicine and 4Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; 5Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Introduction Lungs play an important role in the body’s defense against a variety of pathogens, but this network of immune system mediated defense can be deregulated during acute pulmonary infections. Tropical Medicine, Mahidol University, Bangkok, Thailand; 6Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, UK Objective The present study compares the acute lung inflam- mation (ALI) occurring during Klebsiella pneumoniae B5055- induced pneumonia and sepsis in BALB/c mice. Critical Care 2009, 13(Suppl 4):P1 (doi: 10.1186/cc8057) Methods Pneumonia was induced by intranasal instillation of bacteria (104 CFU) while sepsis was developed by placing the Introduction Urokinase receptor (uPAR, CD87), a glycosylphos- protein, is considered to play an fibrin–thrombin clot c