泛素结合酶E2-EPF高表达质粒的构建及对绒癌细胞JEG-3生长功能的初步探讨.pdfVIP

156 中日友好医院学*2011 年第 25 卷第 3 期 Jourrwl 01 Chi阳-Jap(JJI Friendship Hospital ,2011 Jun , Vol.25 ,No .3 E理FdEi黠坷 泛素结合酶E2-EPF 高表达质粒的构建 及对绒癌细胞JEG-3 生长功能的初步探讨 梁静1.2 ，一F 关璐F (1.北京协和医学院研究生院，100730;2. 中日友好医院妇产科，北京 100029) 摘要 目的:构建泛索结合酶 E2-EPF 高表达质粒，初步探讨 E2-巴PF tE绒癌细胞增殖中的作用。方法:基因克 除技术构建 pcDNA (3.1 +)-E2-EPF 高表达质粒.通过瞬时转染将质粒导人绒癌细胞 JEG-3 中，使 E2-EPF 商 表达;流式细胞仪检测及细胞增殖实验初步研究巴2-EPF 商表达对绒癌细胞生长速率的影响。结果:E2-EPF 商 表达质粒构建成功，瞬时转染后细胞 E2-EPFmRNA 升高约 8.4倍，且G-3 细胞的生长速感显著高于对照组(P 0.05) ，处于 G2-M 期和 S 期细胞显著增加。编论:E2-EPF 参与细胞的生长调控，促进 E2-EPF 表达可以加速绒 癌细胞JEι3 的生长速率。 关键诩:泛素结合酶凹-EPF 质粒;绒癌细胞 JEG-3; 细胞增殖 õ 中图分樊号:R737.33 文献标识码:A 文意编号:1001由0025(2011 )03叶156叶4 doi: 10.3969/j.issn.lωl斗025.201 1.03.∞8 Co阳衍uction of ubiq刨出吨。nj吨叫咱 e皿庐山 E2-EPF high 吨xprωSÎoo plasmid and i也 et1ects 00 growth rate of choriocarcinoma ceU Une JEG-3.川JIANG Jing ,BIAN Mei-lul/Journal of China-Japan Friendship Hωpital ， 20l1 Jun ,25(3) :156-159 Abstract Objec创刊:To construct the E2血EPF over expression plasmid vector and investigate the role of 应2- EPF in cell prolifer百tion and cell cycle of choriocarcinoma cell line JEG-3.Methods:Full len.抽 of E2-EPF was cloned into vector pcDNA (3 .1 +).Cell cycle and growth rate were analysed by flow cytometry and cell proliferation assay respectively 10 study the effect of E2 -EPF high -expression in JEG -3 by transient transfection.Rωu1ts 占2-EPF over-expression plasmid wωsuccess缸lly constructed and the expression level of E2-EPF mRNA was increased 10 about 8.4 fold transit transfection.The growth rate of E2-EPF high …expressed cells were significantly accelerated compare with control (P0.05) ,cells in S and G21M phase were increωed too.Conclusion: High 叩ex