原癌基因产物Ras及cerbB2在着床前小鼠胚胎中的表达_0.docVIP

原癌基因产物Ras及cerbB2在着床前小鼠胚胎中的表达 　　摘要：目的观察小鼠着床前的胚胎细胞中原癌基因ras编码的蛋白产物p21Ras及cerbB2的表达,并了解其对着床前的胚胎发育的可能作用。 方法 采用免疫组织化学ABC法，检测着床前小鼠胚胎中p21Ras及cerbB2蛋白的表达。结果小鼠着床前的胚胎细胞中p21Ras的免疫反应呈阳性,阳性物质分布于胞质中,胚胎外透明带呈阴性反应；而在各期着床前的胚胎中均未检测到cerbB2蛋白的表达。结论小鼠着床前的胚胎中，有原癌基因ras编码蛋白的表达,p21Ras是增殖和分化受体介导的信号传导途径中的重要组成部分，可能在小鼠着床前的胚胎发育过程中起重要的调节作用。CerbB2基因对于围着床期子宫的生长发育具有重要的作用；而在小鼠着床前的胚胎发育过程中似乎不起重要作用。 　　 Expressions of p21Ras and c erbB2 protEins in mouse 　　prEImplantations embryos 　　Abstract: Aim To investigate the expressions and possible role of p21Ras and c erbB2 proteins in preimplantation mouse embryos. Methods Detection of p21Ras and c erbB2 protein expressions by immunohistochemical ABC staining. Results Protein p21Ras was found in preimplantation mouse embryo from 4 cell stage to blastocyst stage. The positive staining substances were located in cytoplasm. Protein c erbB2 was not dectected in any stages of preimplantation mouse embryos. Conclusion Products of proto ;oncogene p21 ras present in mouse preimplantation embryos. It suggests that p21Ras may play a role in early embryo development.  erbB2 proteins in preimplantation mouse embryos. Methods Detection of p21Ras and c erbB2 protein expressions by immunohistochemical ABC staining. Results Protein p21Ras was found in preimplantation mouse embryo from 4 cell stage to blastocyst stage. The positive staining substances were located in cytoplasm. Protein c erbB2 was not dectected in any stages of preimplantation mouse embryos. Conclusion Products of proto oncogene p21 ras present in mouse preimplantation embryos. It suggests that p21Ras may play a role in early embryo development. 　　Keywords: ras gene; c erbB2 gene; embryo; immunohisto;chemical staining ▲ 　　从受精卵卵裂分化到形成成熟的个体，细胞中的某些原癌基因在某一特定的阶段处于一定的活化状。在生理情况下，它们的表达产物在细胞的生长、分化和信号转导等方面起着极为重要的作用。就 目前 的资料看来，绝大多数原癌基因的表达开始于胚胎时期，其中多数主要在胚胎中期开始表达。近年来，国外有人 研究 发现，在着床前的小鼠胚胎中，可检测到一些原癌基因的表达，这些原癌基因对于着床前胚胎的发育起着重要的作用〔1〕。ras基因的产物p21Ras是一种G蛋白，为酪氨酸激酶的下游分子，在酪氨酸激酶信号转导途径中发挥作用。有人在小鼠囊胚期曾检测到p21Ras的表达，但在更早期的胚胎细