柿叶黄酮对离体心脏缺血再灌注损伤的干预作用.docVIP

柿叶黄酮对离体心脏缺血再灌注损伤的干预作用 作者：孙懿，谭宏棣，蓝晓步，黄建春，黄仁彬 【摘要】 目的观察柿叶黄酮（PLF）对大鼠离体心脏缺血再灌注损伤的干预作用。方法48只Wistar雄性大鼠随机分为正常对照组，心肌缺血再灌注组，PLF高、中、低剂量组，阳性药对照组。离体大鼠心脏采用Langendorff法灌流，停灌30 min再灌30 min造成心肌缺血再灌注损伤模型。观察PLF各组对冠脉流出液中天门冬氨酸转氨酶（AST）、肌酸激酶(CK)、乳酸脱氢酶(LDH)活性以及心肌组织中超氧化物歧化酶(SOD)、Na+-K+-ATP酶、Ca2+-ATP酶活性和丙二醛(MDA)含量的影响。结果PLF高、中剂量均可显著改善缺血－再灌注所致的心功能损伤，减少AST 、CK和LDH的释放以及心肌组织MDA的产生，增加SOD、Na+-K+-ATP酶和Ca2+-ATP酶的活性（P0.01或P0.05）。结论PLF对心肌缺血－再灌注损伤具有保护作用, 其机制可能与提高SOD活性、抗氧自由基、减少脂质过氧化反应有关。 【关键词】 柿叶黄酮； 缺血再灌注； 自由基 　　Abstract：ObjectiveTo observe the preventive effect of Persimmon leaf flavones（PLF） on rat myocardial ischemic and reperfusion (I/R) injury. Methods48 Wistar male rats were randomly divided into control, I/R group, high-, medium- and low-dose PLF groups, and verapamil (Ver) group. The isolated perfused rat hearts were set up by Langendorff-perfusion system. Myocardial ischemia reperfusion injury was induced by 30 minutes global ischemia followed by 30 minutes reperfusion. The activities of aspartic transaminase（AST）, creatine kinase (CK), lactate dehydrogenase (LDH) in the effluent from aeteria coronaria were measured to evaluate the injury of myocardial cells and the protective effect of PLF. Meanwhile, the activities of superoxide dismutase (SOD), Na+-K+-ATPase and Ca2+-ATPase, and the content of malondialdehyde (MDA) in myocardial tissues were determined.ResultsHigh- and medium-dose PLF could obviously recover cardiac function, reduce the releases of AST, CK, LDH from I/R rat hearts, increase the activities of SOD, Na+-K+-ATPase and Ca2+-ATPase, and decrease the MDA product (P0.01 or P0.05). ConclusionPF may offer myocardial protective effect against I/R injury. The mechanism may be related to attenuating free radicals. 　　Key words：Persimmon leaf flavones; Ischemia-reperfusion; Free radical 　　心肌缺血再灌注损伤是指心肌缺血一定时间再重新恢复血液供应后,心肌不一定都会恢复其正常功能和结构,反而出现心肌细胞损伤加重的表现,如心律失常、出血性坏死、梗塞面积扩大、心功能低下等现象。目前认为，其发生机理主要与再灌注后细胞内Ca2+超载、氧自由基大量生成、微血管内皮细胞损伤等有关［1］。在我国，柿叶资源非常丰富，柿叶中的黄酮类成分为作用于心血管系统的主要有效成分［2］。近年来,有关黄