旋覆花属8种药用植物ITS2序列分析.PDF

旋覆花属 8 种药用植物 ITS2 序列分析 1 1 2 2 3* 刘义梅 ，喻 珊 ，罗 ，姚 辉 ，陈科力 1. 湖北中医药大学，湖北 武汉 430065 2. 中国医学科学院北京协和医学院 药用植物研究所，北京 100193 摘 要：目的 为旋覆花属8 种药用植物的分子鉴定提供依据。方法 本研究对4 种旋覆花属药用植物的8 个样本ITS2 序 列进行PCR 扩增和测序，同时从GenBank 下载 13 个样本。用MEGA5.0 计算其种间、种内的K2P 距离，各序列变异位点 并进行聚类分析。结果 旋覆花属8 种药用植物ITS2 的长度为210～212 bp ，变异位点为60 个；旋覆花药材2 种不同来源 药用植物有3 个变异位点，与其他同属6 种植物有 13～43 个变异位点；构建的系统发育树显示旋覆花药材的不同基原样本 各聚为一支，能很好与其他6 种植物区分；旋覆花Inula japonica 、欧亚旋覆花I . britannica 、总状土木香I . racemosa 、土木 香I . helenium4 个物种的遗传距离值远小于与羊耳菊I. cappa、赤茎羊耳菊I. rubricaulis 、显脉旋覆花I. nervosa 、泽兰羊耳菊 I. eupatoerioides 4 个物种的遗传距离值。结论 ITS2 序列能够为旋覆花属8 种药用植物的鉴定和Flora of China 对羊耳菊、赤 茎羊耳菊、显脉旋覆花、泽兰羊耳菊分类修订提供一定的分子证据。 关键词：旋覆花属；变异位点；ITS2 序列；遗传距离；分子鉴定 中图分类号：R282.12 文献标志码：A 文章编号：0253-2670(2014)03 - 0 - 05 DOI: 10.7501/j.issn.0253-2670.2014.03. ITS2 sequence analysis of eight medicinal plants in Inula L. 1 1 2 2 1 LIU Yi-mei , YU Shan , LUO Kun , YAO Hui , CHEN Ke-li 1. Hubei University of Chinese Medicine, Wuhan 430065, China 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Science Peking Union Medical College, Beijing 100193, China Abstract: Objective To provide evidence for the molecular identification of eight medicinal plants in Inula L. Methods Eight ITS2 sequence samples of four species in Inula L. were PCR amplified and sequenced, and 13 samples from the GenBank were downloaded. The intra-specific and inter-specific K-2P distances were calculated, and cluster analysis was constructed by MEGA 5.0. Results The length of ITS2 region varied from 210—212 bp with 60 variable sites. Inulae flos from two different sources have three variable sites, and its sibling species had 13 to 43 mutations; Different samples of Inula japonica and I . britannica were gathered together and could be distinguished from its sibling species by UPGMA