心肌酚胧厚 神经递质.docVIP

荞麦花叶总黄酮对压力负荷大鼠脑神经递质的影响 储金秀 韩淑英 朱丽莎 白静 杨瑞娴 （华北煤炭医学院药理学教研室 唐山 063000） 【摘要】目的 探讨荞麦花叶总黄酮（TFBFL）对压力超负荷大鼠脑内神经递质5-羟色胺（5-HT）、去甲肾上腺素（NA）及乙酰胆碱（Ach）的影响和意义。方法 药物干预组分别予TFBFL高剂量（0.4g·kg-1·ｄ-1）、低剂量（0.2g·kg-1·d-1）、卡托普利（60mg·kg-1·d-1）灌胃（ig），模型对照组和假手术组给予等量的生理盐水，每日1次(o.d.)，连续给药1周后用银夹造成腹主动脉狭窄从而导致大鼠心脏超负荷，于手术后1小时、3小时、12小时立即取脑组织，用分光光度法测定脑组织内神经递质NA、5-HT和Ach的含量。结果 手术后1小时、3小时、12小时模型对照组5-HT及NA的含量相对于正常对照组均有较显著的升高（P0.05 ，P0.01），升高的程度随手术时间的延长而增加。TFBFL高低剂量均能降低5-HT和NA的含量，以高剂量作用更强。各组Ach含量改变不显著。结论 TFBFL对压力超负荷大鼠脑内Ach的影响不明显，但能显著降低5-HT和NA 的含量，这可能是TFBFL抑制心肌肥厚的机制之一。 【关键词】荞麦；总黄酮；5-羟色胺；去甲肾上腺素；乙酰胆碱；心肌肥厚 Effects of Total Flavones of Buckwheat Flowers and Leaves on the Neurotransmitters in Brains of Pressure Overload Rats Chu Jinxiu，Han Shuying, Zhu Lisha, Bai Jing, Yang Ruixian （Department of Pharmacology, North China Coal Medical College, Hebei, China 063000） 【Abstract】Objective To explore the influence and significance of total flavones of buckwheat flowers and leaves (TFBFL) on the neurotransmitters i.e. 5-hydroxytryptamine (5-HT), noradrenaline (NA), acetylcholine (Ach) in brains of pressure overload rats. Methods Rats were given TFBFL (0.4g·kg-1·ｄ-1、0.2g·kg-1·d-1) and captopril （60mg·kg-1·d-1） by intragastric administration （ig）in drug interference groups once daily (o.d.) for 1 week, meanwhile, normal saline of the same volume was administrated to the rats in model control group and sham-operated group. Then, cardiac pressure overload model was set by coarctating the abdominal aorta with a silver clip. The brains were collected at 1h, 3h and 12h after operation and homogenated, and the contents of 5-HT, NA and Ach were detected with the spectrophotometric method. Results Compared with normal control group, the content of 5-HT and NA in model control group at 1、3、12 hours after operation increased remarkably （P0.05 ，P0.01） and showed time dependence. TFBFL at the two dosages could both lower the content of 5-HT and NA and showed dosage dependence. However, the content of Ach had no significant chan