DU_730紫外_可见分光光度计应用研究.pdf

第4期2010年8月 现代科学仪器 No.4 Aug. 2010 Modern Scientific Instruments 141 DU-730紫外/可见分光光度计的应用研究 张燕婉　叶珏　宋莉　孟宪敏 ( 中国医学科学院阜外医院中心实验室 北京 100037) 摘　要　发表此文章的目的是向读者介绍一台国际先进水平的光谱仪器—美国 BECKMAN( 贝克曼 ) 公司生产的 DU-730 紫 外 / 可见分光光度计 , 它性能优良、应用广泛、操作简便 , 是生物医学实验室中非常实用的计量仪器。本文首先阐述了 DU- 730 紫外 / 可见分光光度计的结构和原理 , 特别是这台仪器具有的先进的“微聚焦光束技术”。然后，介绍了仪器的各种主要 技术参数 , 重点讨论了仪器的功能和所涉及的应用领域 , 包括 : 全波长扫描、固定波长测定、单一组分分析和蛋白质核酸的 生物学测定等。最后作者为加强仪器的管理阐述了仪器的正确使用方法与维护 , 为正在应用这台仪器的读者提供一些有用的 经验。 关键词　紫外/可见分光光度计;微聚焦光束技术;原理;应用;使用与维护 中图分类号　TH744.12 1 1 1 1 Zhang Yanwan ,Ye Jue , Song Li , Meng Xianmin (Core Laboratory of Fuwai Hospital for Cardiology,Chinese Academy of Medical Sciences, Beijing 100037,China) Abstract The objective of this paper is to introduce a spectral instrument with international advanced level –DU730 UV/Vis spectrophotometer from BECKMAN Inc. This instrument is provided with excellent property, extensive applica- tion and convenient manipulation, and is measuring instrument practically in biomedical laboratory. Firstly this paper had elaborated the structure and principle of DU730 UV/Vis spectrophotometer, and “ advanced technique of micro-focused beam” particularly. Still this paper introduced chief technical parameter of the instrument. The function and applied fi eld of this instrument was discussed weightily including wavelength scan, determination of fixed wavelength, single componet analysis, biological determination of protein/nucleic acid and so on. Finally the correct manipulation and maintenance of DU730 UV/Vis spectrophotometer had been elaborated to intensify instrumental management. The authors have provided the helpful working experience for users with this spectrophotometer. Key words UV/Vis spectrophotometer