最详细：CRISPRCas9系统原理应用及发展.pptVIP

3.2 sg-RNA：Cas介导基因修饰 2013年1月29日在《nature biotechnology》上发表的《efficient genome editing in zebra fish using a CRISPR-Cas system》一文中，作者利用人工合成的sgRNAs能指导Cas9内源性核酸酶对斑马鱼胚胎基因进行修饰。 3.2 sg-RNA：Cas介导基因修饰 3.2 sg-RNA：Cas介导基因修饰 * 中山大学生命科学学院 Like cruise missiles attacking military targets, these proteins, called transcription activator–like effectors (TALEs), can be programmed to home in on specific DNA sequences and carry out an action once there. Each TALE repeat targets a specifi c DNA base: A, T, C, or G. The repeats are the same series of 34 animo acids (color-coded) except at positions 12 and 1