基于微生物发酵床养猪垫料的生物肥药特性.doc

微生物发酵床养猪垫料生物肥药特性研究 欧阳江华1，刘波1*，郑雪芳，林抗美，车建美1， (福建省农业科学院农业生物资源研究所，福建福州350003) 摘 要：以利用两年后废弃的微生物发酵床养猪垫料为发酵原料，进行垫料好氧发酵试验，探讨添加了生防菌剂ANTI-8098A后生物肥药的特性。首先进行一级液体发酵，发酵出108cfu/g的ANTI-8098A种子液2升，然后把种子液稀释100倍，以1：10的重量比与垫料混合均匀，调节含水量为50%左右，进行二级固体发酵，同时设置空白对照。然后按第2、4、8、16、32天取样5次，分析生物肥药的特性。结果显示：接种生防菌ANTI-8098A菌剂的生物肥药与未接种菌剂的生物肥药理化性质差异不显著，但接种生防菌ANTI-8098A菌剂的生物肥药中速效氮源下降很快。在微生物群落结构变化中，真菌数量变化和放线菌数量变化差异不明显，接种生防菌ANTI-8098A菌剂的生物肥药活菌量达到了1.7×108cfu/g，符合农业部有关肥料的质量标准，并且接入的生防菌成了优势群落。 关键词：ANTI-8098A；养猪垫料；生物肥药；微生物群落；理化性质； Characteristics of microbial fertilizer using the stroma cusion material from non-pollution pigsty Abstract: An aerobic fermentation test was conducted using the abandoned stoma cushion in the non-pollution pigsty to study the characteristics of the bio-fertilizers after addition the ANTI-8098A microbial community. Firstly, two liters of seed solution was abtained by the first liquid fermentation, then the seed solution was diluted 100 times to weight ratio of 1:10 mixed with the stoma cushion, adjust water content of about 50% for second solid fermentation, while setting the control. And then sampled by the first 5 days 2,4,8,16,32, analyze the characteristics of bio-fertilizer. The results indicated that the physical and chemical properties of biological fertilizer after ANTI-8098A Inoculation were no significant differences with the control, However, the available nitrogen of bio-fertilizer after ANTI-8098A inoculation decreased rapidly. Microbial community structure changes in the number of fungi and actinomycetes were no obvious differences, but the number of ANTI-8098A effective bacteria amounts to 1.7×108cfu/g,the number measure up the standard of bio-fertilizer of Department of Agriculture of China,while ANTI-8098A strain became the dominant species. Key words: ANTI-8098A; stoma cushion; bio-fertilizer; microbial community; physical and chemical properties 微生物发酵床微生物发酵床作者利用对青枯雷尔氏菌具有较强的抑菌作用[3-5]生防菌蜡状芽孢杆菌ANTI-8098A菌株由福建省农业科学院生物农药研究中心自番茄植株根际土壤分离获得，对茄子青枯病的防效在75%-85%，菌株经德