Biolog接种液配制方法(国外英文资料).doc

Biolog接种液配制方法 Preparation of inoculated fluid 1, GN/GP - IF (gram-negative bacillus) Formula is as follows: 0.40% sodium chloride (NaCl), sodium chloride (to maintain osmotic pressure) 0.03% Pluronic f-68 (e.g., Sigma # P7061) polyether f-68 (a non-ionic surfactant, which reduces the surface tension and makes it easily dispersed in water) 0.02% Gellan Gum (Phytagel? Sigma # P8169) gelatine (a kind of edible glue that increases the viscosity of the liquid, and spreads the bacteria evenly) The preparation process is as follows: Add 0.2 grams of Gellan Gum to 1 liter of water; Boil and keep stirring until the Gellan Gum is dissolved. Stop heating and stir. Add 4 grams of NaCl, stirring until completely dissolved. Add 0.3 grams of ether and f-68, stirring until completely dissolved. In a 20-by-150mm tube, each tube is about 19mL; 7. Sterilization 30 minutes under 121 ℃, and set aside. AN -if (anaerobic method) Add 750ml of purified water to a liter of heat-resistant glass container, and then add the following reagent: 3.0 g of NaCl NaHCO3 0.63 g 0.225 g Pluronic f-68 polyether f-68 (a non-ionic surfactant, which reduces surface tension and allows the bacteria to be easily dispersed in water) 0.15 g Phytagel Place the glass container on the heating plate. The lid is slightly loose and begins to stir at low speed. Turn on the heating switch. Heat will boil the solution. The solution will be clarified. Heat until the foam reaches the bottleneck. Tighten the lid, remove the container from the heating board, and immediately place it in a high pressure sterilizer for 30 minutes. 3. When the autoclave cooled to 80-90 ℃, opening the autoclave to tighten the cover again. Then immediately put the glass container in the anaerobic incubator. In anaerobic incubator, rotate the lid. The pump attached to the pump will pump the gas in the tank into the solution and blow the solution until the foam reaches the glass containers neck (or about 20 minutes). Add 1.125 ml 1% of the sodium t