发展曲线测定(国外英文资料).doc

生长曲线测定 1 purpose Understanding the characteristics and principle of bacterial growth curve The growth curve of bacteria was measured by means of turbidity 2 the principle of A small amount of bacteria inoculated to a certain volume, suitable for fresh medium, in appropriate conditions for culture, timing determination of the bacterial inoculum amount, to logarithmic ordinate, the amount of bacteria growth time as abscissa, draw the curve of the growth curve. It reflects the pattern of population growth of single cell microorganism in a certain environment under certain environmental conditions. According to the different growth rate, the growth curve can be divided into delay, logarithmic stage, period of stability and decline. The length of these four periods varied with the heritability, the amount of inoculation and the condition of the culture. Therefore, by measuring the growth curve of microorganism, it is important to understand the growth regularity of the bacteria and have important guiding significance for scientific research and production. There are many different methods for determining the number of microorganisms, which can be chosen according to the requirements and laboratory conditions. Turbidimetric method is applied in this experiment, due to the concentration of the bacteria suspension is proportional to the optical density (OD value), so use spectrophotometer determination of bacteria suspension optical density to infer bacteria liquid concentration, and measured the OD value the cultivation of the corresponding time drawing, can draw the bacteria growth curve in certain conditions, this method fast and easy. 3 material 3.1 strains E. coli 3.2 culture medium The meat cream peptin medium Instruments and instruments 721 spectrophotometer, better than color glass, thermostat table, sterile straw, test tube, triangle bottle. 4 process Seed liquid, label, inoculation, culture, test Five methods 5.1 seed preparation The bacteria of the escherichia c