高等植物液泡分离研究进展.pdf

2003, 23( 12) : 22 162222 A cta Bot . Bor eal . -Occid ent .Sin . : 1000-4025( 2003) 12-22 16-07 X 门中华, 李生秀 ( , 712100) : . , . , . : ; ; ; : Q944. 6: A Progress i n isolation of vacuol es of higher pl ants M EN Zhong-hua, LI Sh ng-x iu ( , - , , Coll g of Natural R sourc s and Environm nt Sci nc Nor thw st Sci T ch Univ rsity of Agricultur and For stry Yang ling Shaanxi 712100,China) Abstract: T h vacuol s of plant c lls ar multifunctional organ ll s. R s arch on isolation of matur vac- uol s of high r plants has mad substantial pr ogr ss in th w orld. A t pr s nt, s v ral ff ctiv m thods of , , , , . isolation has b n d v lop d such as osmotic lysis polybas induc d lysis machin lysis and so on Bas d on our pr liminary inv stigation and lit ratur , a compr h nsiv r vi w on achi v m nts of isolation of ma- tur vacuol s of high r plants both in China and oth r countri s in r c nt y ars has b n mad in this arti- cl . : ; ; ; Key words plant matur vacuol isolation m thod pr ogr ss , . 1998 , L igh Sand rs [ 3] , ( c n- . [ 1] tr al vacuol ) , 90% ; , , , . . 20 70 . ,