高剂量苏氨酸锌和硫酸锌对大鼠胚胎-胎仔发育毒性的比较.pdfVIP

※营养卫生 昌自融事 2014, VoJ. 35, NO.17 241 高剂量苏氨酸伴和硫酸铐对大鼠胚胎·胎仔 发育毒性的比较 冯建萍，王光然，胡晓波，姜雯雯，谢明勇* (南昌大学食品科学与技术国家重点实验室，江西南昌 33∞47) 摘 要z 目的:在胎鼠器官形成期6~15 d对怀孕的Sprague-Dawley (SD) 大鼠连续灌胃高剂量铸源，观察苏氨 酸辞和硫酸钵对SD大鼠胚胎.胎仔发育毒性。方法z 将苏氨酸铮按1 200 mg/ (kg • d) 的剂量(以体质量计，下 同) ，硫酸铮则给予相同剂量的镑 (575 mg/ (kg • d) ) ，于娃振第6~15天连续灌胃给药，观察孕鼠基本生活 状况。每隔3d称1 次体质量，娃振第20天处死孕鼠，记录子宫质量、着床数、死胎数、胎盘质量及胎鼠体质量 等，观察活胎仔外观异常与否。每窝112 胎鼠放入Bouins液固定两周作内脏检查，另112 胎鼠作骨路畸形检查。结 果:苏氨酸镑和硫酸钵组部分孕鼠给药初期均出现食欲低下，毛发不顺等，且摄食量明显降低 (P0.05) ， 实 验结束时孕鼠体质量增加显著减少 (P0.05) 。苏氨酸铸组有18 例胎鼠胸骨缺失，与玉米油组比较有显著差异 (P0.05) 。硫酸铮组有13 例胎鼠胸骨缺失，同时有2 只孕鼠死亡，与玉米油组比较有显著差异 (P0.05) 。结 论z 两种高剂量的铸源对SD孕鼠都有一定的生殖毒性，但苏氨酸铸相对比较安全。 关键词:苏氨酸铸:硫酸铮:骨髓畸形:发育毒性 Comparison of Teratogenic Effects of Different Zinc Sources at High Dose on Fetuses of Sprague-Dawley (SD) Rats FENG Jian-ping, WANG Guang-r缸， HU Xiao-bo, nANG Wen-wen , XIE Ming-yong* (State Key Laboratory of Food Science and Technology, Nanchang Univers坷， Nanchang 33∞47， China) Abstract: Objective: To observe the toxic effects of zinc threoninate chelate (Thr-Zn) and zinc sulfate on the embryo-fetal development in Sprague-Dawley (SD) rats on 由ys 6 to 15 of pregnancy when they were diluted with com oil. Methods: The pregnant rats were administered daily with Thr-Zn at a dose of 1 2∞mgl(kgd) bw or 由es缸ne zinc dose 仕om zinc sulfate (575 mgl(kgd)) by gavage during days 6 to 15 of pregnancy and the basic living conditions were observed. Body weights were measured on days 0, 4 , 8, 12, 16 and 20 of pre伊ancy， respectively. On day 20, the pre伊ant rats were sacrificed and the让 uterus were collected and weighed. The numbers of corpora lutea, i