鼻咽癌细胞辐射抗拒的比较蛋白质组学分析.pdfVIP

中华放射民学与防护杂志 2012 年 6 月第 32 警第3 期 Chìn J Radíol M.d Prol ,June 2012 ,Vol. 32 ,No. 3 .245 ·放射生物学· 鼻咽癌细胞辐射抗拒的比较蛋白质组学分析 朱小东黄诗婷曲颂苏芳郭亚王金子 l 摘要1 目前 通过分离并鉴定具有不同辐射抗拒的人鼻观癌细胞株 CNE-2R 与 CNE号的差 异表达蛋白.探讨与鼻咽癌细胞辐射抗拒相关的分子机制。方法 分别提取鼻咽癌辐射抗拒细胞 株 CNE-2R 及其亲本细胞株 CNE-2 的总蛋白，采用双向凝胶电泳分离，经软件分析识别差异表达蛋 臼点，应用基质辅助激光解析电离飞行时间串联质谱技术( MALOI.TOF-MS) 鉴定差异蛋白质。结果 两种不同辐射敏感性的鼻唰癌细胞株 CNE-2R 和 CNE-2 中，共筛选出差异表达明显的蛋白质点有 :2 个.其中 11 个蛋白质被鉴定成功，在 CNE-2R 中上词的蛋白有 3 个， r调的蛋臼有 S 个。结论 不同辐射敏感性的鼻咽癌细胞的差异表达蛋臼，主要涉及调节凋亡、DNA 损伤与修复、细胞周期调 控、RNA 转录、细胞信号转导、细胞骨架组成及辐射应激反应等多个方面。 I 关键词1 鼻咽痛s 辐射抵抗: 比较蛋白质组学 Comparative proteomlc anaJysls 00 radioreslstaot oasopharyngeaJ carCÎooma ceJJ ZHU Xiao- dong ,HUANG Shi-ting , QU Song ,SU Fang ， ιUO Ya , WANG Jin-.i. Deparlm.nl 0/ Radiolh.rapy , Canc.r Hospilal 01 Guangxi M.d阳al U刑versilY ， Cancer lnslilule 01 Guangxi Zhuang A Ulonomous Region , Nanning 53ω21 ， China Corresponding aulhor: ZHU Xiao-dong .Email:zhU%iaodongI966@ [ Ab.tra~t 1 Objective To discover radioresistance-a..ociated proteins by perfonning comp町al1ve proteomic analysÎSi on nasophllryngeal carcinoma cell line8. Methods The lota] proteins were extracled from radioresistanl human nasopharyngeal carcinoma cell line CNE-2R and ito parenlal cell line CNE-2 , respeclively. These proleins were aep.rated hy high qualily two-dimensional polyoCIγlamide gel electrophoresis (2-DE) and lhen the 2-DE profiles were screened for differenliolly expressed protein opols by the Image Master 5.0 software. Those spots were identified by a malrix.ossisted I.oer desorplionl ionization time-of-flight tandem m.ss spectrometry. ResuJts 32 剖gnl白cantly differentially expre8ed protein pOIS were ocreened in two differenl radio.en.itivity ceJJ lines and 11 protein. were identified by tandem mass spectromet町， among which 3 proteins were up-regulaled in radioresistant human n