均匀设计法优化桦树单宁超声波辅助提取工艺.pdfVIP

资源与环境科学 现代农业科技 2012 年第 23 期 均匀设计法优化桦树单宁超声波辅助提取工艺 崔海峰 l 郭盛磊 1 ， 2 于涛l 丁为民 1 ， 3 阎秀峰 1 * (东北林业大学盐碱地生物资源环境研究中心东北油田盐碱植被恢复与重建教育部重点实验室，黑龙江哈尔滨 15ω40; 2 黑龙江中医药大学药学院; 3 哈尔滨理工大学化学与环境工程学院) 摘要 白桦树皮是白桦采伐与加工剩余物，是重要的生物质资源。利用均匀设计法优化了超声波辅助提取白桦树皮中单宁的工艺。超 声波辅助提取桦树羊宁的最佳条件为:乙醇浓度 159毛 (v/v) ，提取时间 30min ，提取温度 35 t::，超声波功率 500W; 此条件下提取桦树单宁， 收率达到 6.44%，纯度为 45.689毛。与传统的水提法、水/乙醇提取方法相比，超声波辅助提取法具有提取时间短、提取率高的优点，具有较好 的应用前景。 关键词 白桦树皮;单宁;均匀设计:超声波:提取工艺 中图分类号 TQ654.2 文献标识码 A 文章编号 1007-5739(2012)23-0222卢02 Optimization of Ultrasound-assisted Extraction Process of Tannins from White Birch Bark Using Uniform Design CUI Hai-feng 1 Guo Sheng-lei 1,2 YU Tao 1 DING Wei-min 1,3 YAN Xiu-feng l ( Alkali Soil Natural Environmental Science Center ,Northeast forestry University ,Key Laboratory ofSaline-alkali Vegetation Ecology Restoration in Oil Field (Ministry of Education) , Harbin Heilongjiang 150040; 2 College of Pharmacy ,Heilongjiang University of Chinese Medicine; 3 School of Chemical and Environmental Engineering , Harbin University of Science and Technology) Abstract White birch (Betula plαtyphyllα) bark ,one of the important biomaterial resources ,is the remains after cutting and processing of the tree. Ti}e ultrasound -assisted extraction process of tannins from white birch bark were optimized using uniform design , The optimal conditions were 15% alcohol (v/v) as the extraction solvent ,30 min as the extraction time ,and 35t:: as the extraction temperature , 500 W of ultrasound power. Tannin yield could reach up to 6.449奋 with the purity of 45 , 68 % under above conditions. Comparing with conventional water and water/ethanol extraction , ultrasoundassisted extraction method showed better utilization prospect with shorter extraction time and bette