VEGF和Ki67在膀胱移行细胞癌中的表达和临床意义.docVIP

VEGF和Ki-67在膀胱移行细胞癌中的表达和临床意义 　　作者：杨宏,雷永虹,秦扬,他同生　　作者单位：昆明医学院第三附属医院泌尿外科 　　【摘要】 目的　探讨膀胱移行细胞癌(BTCC)组织中血管内皮生长因子(VEGF)和Ki-67的表达和临床意义，研究二者与肿瘤血管形成和肿瘤细胞增殖的关系。方法　采用免疫组织化学SP法检测68例BTCC组织中VEGF和Ki-67的表达，分析VEGF和Ki-67表达与肿瘤病理分级、复发、转移及预后的关系。结果　BTCC组织中VEGF阳性率为73.53%，Ki-67阳性率为70.59%。在不同病理分级BTCC组织中VEGF和Ki-67的表达有明显差异，病理分级高的明显高于分级低的(P0.05);浸润性肿瘤明显高于表浅性肿瘤(P0.05);复发组均明显高于无复发组(P0.05);Ki-67和VEGF协同强阳性表达率差异有统计学意义(P0.01)。结论　VEGF和Ki-67的阳性表达尤其是联合阳性表达对判断膀胱癌复发、评估预后具有重要意义。 　　【关键词】 膀胱肿瘤;表皮生长因子;Ki-67抗原 　　【Abstract】　Objective　To investigate the expression and clinical significance of vascular endothelial growth factor(VEGF) and Ki-67 in bladder transitional cell cancer(BTCC)and to explore the correlation between VEGF and Ki-67 in the angiogenesis of tumor and tumor cell proliferation.Methods　The expressions of VEGF and Ki-67 were detected by immunohistochemical methods in 68 cases of BTCC. The correlation between the expression of VEGF and Ki-67 and various clinicopathological parameters including pathological grade，recurrence， metastasis and prognostic evaluation were analyzed.Results　The positive expression rates of VEGF and Ki-67 in 68 cases of BTCC were 73.53% and 70.59% respectively.There was a significant difference in VEGF and Ki-67 expression among differently pathological grade， and the positive rate in high grade was significantly higher than that in low grade(P0.05).The positive rate of the invasive tumor was significantly higher than that in superficial tumor(P0.05).The positive rate in recurrence group was significantly higher than that in non-recurrence group(P0.05). The combined positive rate difference of Ki-67-and VEGF had statistical significance(P0.01). Conclusions　The expression of VEGF and Ki-67， especially the positive combined expression plays an important role in the judgment of recurrence and prognostic evaluation. 　　【Key words】　bladder cancer;vascular endothelial growth factor;Ki-67 　　为探讨膀胱癌中癌基因的表达与其生物学行为的关系，采取免疫组化 SP法对68例膀胱移行细胞癌(bladder transitional cell ca