武汉大学分子生物学讲义（英文）.ppt

* Linked with the termination of transcription The CTD tail is involved in recruiting the polyadenylation enzymes. The transcribed poly-A signal triggers the reactions: Cleavage of the message Addition of poly-A Termination of transcription RNA processing 23’ end polyadenylation * 1. CPSF (cleavage and polyadenylation specificity factor) CstF (cleavage stimulation factor) bind to the poly-A signal, leading to the RNA cleavage 2. Poly-A polymerase (PAP) adds ~ 200 As at the 3’ end of the RNA, using ATP as a substrate Fig 12-24 polyadenylation and termination * Transcription termination * Transcription termination is linked to RNA destruction by a highly processive RNase. Torpedo model: The second RNA molecule without a cap is recognized and degrade by a possessive RNase-?Pol II dissociation from DNA template. Allosteric model: The transfer of the 3’ processing enzyme to RNAP II induces conformational change ? RNAP II processivity reduces ? spontaneous termination. Fig. 12-25 * Topic 4:Transcription by RNA polymerase I and III CHAPTER12: Mechanisms of Transcription * RNA Pol I III recognize distinct promoters , using distinct sets of transcription factors, but still require TBP. Pol I: transcribes rRNA precursor encoding gene (multi-copy gene) Pol III: transcribes tRNA genes, snRNA genes and 5S rRNA genes The transcription in eukaryotes * Pol I promoter recognition Fig 12-26 Pol I promoter region Upstream control element UBF binds to the upstream of UCE, bring SL1 to the downstream part of UCE. SL1 in turn recruits RNAP I to the core promoter for transcription * Fig 12-27 Pol III core promoter TFIIIC binds to the promoter, recruiting TFIIIB, which in turn recruits RNAP III Pol III promoter are found downstream of transcription start site. * RNA polymerases (RNAP, 真核和原核的异同) and transcription cycle (Initiation, elongation and termination) Transcription cycle in bacteria: Initiation: (1) The feature of s70 promoters. (2) Promoter binding by s70 transcr