血小板分离技巧(国外英文资料).docVIP

血小板分离技术(国外英文资料) Platelet separation The basic content Platelet separation technique (Plateletpheresis or platelet - richplasmapheresis) is separate from the whole blood platelet, preparation of rich in platelet plasma (platelet - richplasma, PRP) or concentrated platelet, is an important technology of component blood transfusion. Separation in the early 1990 s, the platelet was transplanted to the extracorporeal circulation (Cardiopulmonarybypass, CPB) in cardiac surgery, the significant blood protection is causing more and more attention. Basic equipment and methods The platelet separation technique USES a machine to separate the blood components from each other. Its basic configuration includes: blood collection piping, centrifuge cup, blood collection bag and machine hardware. Specific operation of platelet separation. In the application of extracorporeal circulation cardiac surgery, is in after the success of the anesthesia via central venous catheter bloodletting, namely venous catheter in vitro open end and blood collection tube directly connected to the machine, anticoagulant citrate salt, phosphate, glucose solution (Citratephosphatedextrose, CPD) in the pipeline and blood are mixed, separate PRP into the blood by the centrifugal machine. The blood is also replenished by the peripheral vein and the fluid in the peripheral veins to maintain the stability of the blood. It is also possible to remove the upper arm vein directly from the central venous catheter to release blood. The blood flow rate should be controlled at 50 to 100ml per minute. According to the difference in machine performance, two to three rounds are collected to collect enough platelets. The rest of the blood is returned to the patient at the end of each cycle to ensure that the patients blood is normal with oxygen and blood dynamics. The entire separation extraction process is completed before the CPB liver. The CPB ends, the fish protein is fully neutralized and the PRP is lost to the pati