利用大孔树脂吸附提取桑黄总黄自同-上海海洋大学学报.PDF

第 16 卷第 1 期 上海水产大学学报 Vo l. 16 , No.l 2007 年 1 月 JOURNAL OF SHANGHAI FISHERIES UNIVERSITY Jan. ,2007 文章编号: 1004 -7271(2007)01 -0060 -04 利用大孔树脂吸附提取桑黄总黄自同 宋钳1 ， 2 周培根王艳杨没2 (1.上海水产大学食品学院，上海 200090;2 上海市农业科学研究院食用菌研究所，上海 201106 ) 摘 要:利用几种大孔树脂对桑黄黄酣的吸附与解吸性能进行了比较筛选实验。结果表明，样品浓度、吸附时 间和解吸时间等因素都会对提取效果有影响。通过比较发现树脂 DM301 比较适合于吸附提取桑黄总黄酬。 应用 DM301 型大孔树脂，样品液的体积与树脂量比为(2 -3): l(mL: g) ，黄嗣浓度为 2 mglmL，吸附时间为 14 h ，用 3 倍于样品液体积的60% 乙醇解吸5 h 可以得到比较理想的提取结果，平均回收率为95.2% 。 关键词:桑黄;黄酣;大孔树脂;吸附 中图分类号:TS 20 1. 2 文献标识码:A Extraction of flavonoids from Phellinus igniarus by adsorption of macroreticular resins l J 2 SONG BOI ,2 ,ZHOU Pei-gen ,WANG Yan , YANG Yan (1. College 01 Food Science ,Shanghai Fisheries University , Shanghai 200090 , China; 2. Edible Fungi Institute ， Shanghaι Academy 01Agricultural Science , Shanghai 201106 , China) Abstract :The adsorption and desorption of flavonoids from Phellinus igniarus by 5 kinds of macroreticular resins were compared. It was found that DM301 resin was more suitable for the adsorptive extraction of flavonoid compounds. When the DM301 was applied to its isolation , the flavonoid product from Phellinus ~gmar山 with a recovery of 95. 2% was obtained. The extraction conditions are as follows: the ratio of the volume (mL) of sample to the mass (g) of resins is (2 - 3 ) : 1; the concentration of flavonoids was 2 mgl mL; the adsorption time was 14 h , and the desorption solvent and the desorption time were 60% ethanol with three times the sample volume and 5 h ,respectively. Key words: Phellinus igniarius; flavonoids