bcr-abl基因疫苗对小鼠SP2-0-bcr-abl移植瘤的影响.docVIP

bcr-abl基因疫苗对小鼠SP2/0/bcr-abl移植瘤的影响 作者：姜扬文，钱莉，蒋桂花，刘伟，龚卫娟，季明春 【摘要】 　　为了 研究 bcr-abl融合基因疫苗对小鼠SP2/0/bcr-abl移植瘤的 影响 ，采用pVbcr-abl 、pVbcr-abl/mIL7两种质粒分别免疫BALB/c小鼠，然后用SP2/0/bcr-abl细胞攻击免疫小鼠，观察疫苗对SP2/0/bcr-abl移植瘤生长的影响，检验其是否具有免疫保护作用。结果表明：用基因疫苗免疫的两个实验组与两个对照组（空白对照组和空载体对照组）相比，在移植肿瘤形成时间、肿瘤表面破溃时间、肿瘤体积、荷瘤生存期等指标上均存在明显差异。pVbcr-abl/mIL7免疫小鼠组的荷瘤生存时间比pVbcr-abl免疫组相对延长。常规病 理学 分析 发现，对照组小鼠的移植瘤组织比较致密，瘤细胞体积较大，形状不规则，而两个免疫组的肿瘤组织较为疏松，肿瘤细胞体积相对较小，并有大量炎性细胞浸润。两个对照组小鼠的肝脏组织内发现有大量肿瘤转移灶，而两个免疫组小鼠则未发现。结论： 接受 bcr-abl 基因疫苗免疫的小鼠被诱导产生较强的特异性免疫保护力，对 SP2/0/bcr-abl 移植瘤的体内生长有一定抑制能力。 【关键词】 慢性粒细胞白血病；bcr-abl融合基因；基因疫苗；SP2/0/bcr-abl移植瘤 　　Influences of bcr-abl Gene Vaccine on Inoculated SP2/0/bcr-abl Tumor Cells in Mice 　　Abstract To study the influence of vaccine of bcr-abl fusion gene fragment on inoculated SP2/0/bcr-abl tumor cells in mice，BALB/c mice were immunized with pVbcr-abl，pVbcr-abl/mIL7 plasmids，respectively，then SP2/0/bcr-abl cells expressing the fragment of bcr-abl fusion gene were inoculated subcutaneously into the groin of BALB/c mice in order to observe the effect of vaccine on growth of inoculated SP2/0/bcr-abl tumor cells. The results showed that there were distinct differences on the time of tumor growth，the time of tumor ulceration，tumor volume and survival time of mice bearing tumor between two immunized groups and two control groups (blank and vacant plasmid groups). The mice immunized with pVbcr-abl/mIL7 lived longer as compared to mice immunized with pVbcr-abl. The tissue of inoculated tumor was more compact，tumor organ was larger，tumor form was irregular in 2 control groups，while the tissue of inoculated tumor was looser，tumor volume was smaller，and with mass inflammatory infiltration in two immunized groups. Moreover， the metastatic tumor cells were found in the livers of control groups，but not observed in two immunized groups. It is concluded that the protection occured in immunized mice which inhibited the growth of SP2/0/bcr-abl tumor cell in vivo. 　　Key words chronic myeloid leukemia；b