HG颗粒对CCl4复合因素致肝纤维化大鼠血清IL-1和IL-6含量的影响.docVIP

HG颗粒对CCl4复合因素致肝纤维化大鼠血清IL-1和IL-6含量的影响 作者：毕红征，章金涛，黄国钧，薛敬礼 【摘要】 　　目的研究HG颗粒对CCl4 （四氯化碳）复合因素致肝纤维化大鼠血清IL-1(白介素1)和IL-6(白介素6)含量的影响。方法70只SD大鼠，除空白对照组10只外，其余各组大鼠皮下注射CCl4，同时予高脂饲料和20%乙醇喂养6周建立肝纤维化大鼠模型，存活大鼠按性别和体重随机分为5组，再加上空白对照组，动物共计6组，分别给予药物或蒸馏水。空白对照组：蒸馏水0.1 ml/kg；模型对照组：蒸馏水0.1 ml/kg；HG颗粒高、中、低 3 种剂量组:6，3，1.5g生药/kg；复方鳖甲软肝片组:1g/kg，灌胃 治疗 ，1次/d，连续4周。观察模型大鼠血清IL-1和IL-6含量的变化。结果模型大鼠血清IL-1和IL-6含量均明显增高。HG颗粒3种剂量组治疗后均能降低模型大鼠血清IL-1和IL-6含量，复方鳖甲软肝片亦可降低大鼠血清IL-1和IL-6含量。结论HG颗粒对CCl4复合因素致肝纤维化大鼠有显著的治疗作用。 【关键词】 HG颗粒 IL-1 IL-6 　　Abstract：ObjectiveTo explore the effects of HGKL on serum IL-1 and IL-6 in HF model rats.MethodsSeventy SD rats excluding ten rats in blank control group were injected carbon tetrar-chloride（CCl4） hypodermicly,and were fed with high fat diet and 20% ethyl alcohol for six weeks to establish HF model rats. Existing rats were devided into five groups randomly basing on sex and wEight.All rats were given drugs or distilled water.Blank control group: distilled water 0.1ml/kg；model control group: distilled water 0.1 ml/kg；HGKL high,middle,low groups：6,3,1.5g crude drug/kg；FFgBJRGP group:1 g/kg,once a day for four weeks.The contents of IL-1and IL-6 in serum were detected. ResultsThe contents of IL-1and IL-6 in model rats were significantly increased as compared with the blank group. After treatment with HGKL high,middle,low dosages，the contents of IL-1 and IL-6 in model rats were decreased markedly，so did FFBJRGP.ConclusionHGKL has obvious effect on HF model rats induced by carbon tetrar-chloride compounded with other factors. 　　Key words：HGKL； IL-1； IL-6 　　肝纤维化(hepatic fibrosis)是各种慢性肝病进展中由于肝内纤维生成与降解失衡，导致过多的胶原在肝内沉积所致，常伴有炎症并可 发展 为肝硬化［1］。肝纤维化是慢性肝病重要的病理特征，也是肝硬化发生的前奏和必经中间环节。阻断肝纤维化的发生和发展，对防治肝硬化和慢性肝病具有重要意义［2，3］。美国著名肝病学家Hans Popper指出：“谁能阻止或延缓肝纤维化的发生，谁将治愈大多数慢性肝病”。HG颗粒是由丹参、桃仁等中药组成的复方制剂，此方来源于知名专家的多年临床经验 总结 ，对肝纤维化有着良好的治疗效果。本实验旨在观察HG颗粒对CCl4复合因素所致肝纤维化大鼠血清IL-1和IL-6含量的影响，探讨其治疗机理，为其临床用药提供实验依据。 　　 1 材料与方法 　　1.1 材料HG颗粒10g生药/袋，成都中医药大学中药制剂学教研室提供，批号：041014。复方鳖甲软肝片0.5 g/片，内蒙古福瑞中蒙药科技有限公司出品，批号：2003071