运动对心肌细胞凋亡及相关基因表达的影响.PDFVIP

安徽农业大学学报, 2013, 40(1): 90-94 Journal of Anhui Agricultural University 网络出版时间：2012-12-29 13:31:27 [URL] /kcms/detail/34.1162.S1331.012.html 运动对心肌细胞凋亡及相关基因表达的影响 蒋 磊，周振茂，夏美燕，杜世全* (安徽医科大学体育教学部，合肥 230032) 摘 要：通过研究不同运动强度对心肌细胞凋亡基因 Bcl-2 、Bax 和 Caspase-3 的影响，探讨了运动对心肌细胞 凋亡的作用机制。将大鼠分为 3 组（正常对照组、一般游泳有氧训练组和力竭性过度训练组）并建立运动模型；采 用实时荧光定量 PCR 技术观察心肌细胞中凋亡基因 Bcl-2、Bax 和 Caspase-3 mRNA 的表达；采用Western blot 方 法观察 Bcl-2 、Bax 、Caspase-3 蛋白表达情况。结果显示，与对照组相比，一般游泳有氧训练组大鼠调控基因 Bcl-2 表达显著增加，对细胞的凋亡起到一定的抑制作用；而力竭过度训练组中心肌细胞相关调控基因 Bcl-2 表达下降， Bax 、Caspase-3 的表达升高促进凋亡。因此推定超负荷的运动会加重大鼠心肌细胞的凋亡，凋亡的发生可能与调 控基因 Bcl-2、Bax 和 Caspase-3 有关。 关键词：运动；凋亡基因；心肌细胞 中图分类号：G804.7; S865.12 文献标识码：A 文章编号：1672−352X (2013)01−0090−05 Effects of exercises on apoptotic and related gene expression in myocardium JIANG Lei, ZHOU Zhen-mao, XIA Mei-yan, DU Shi-quan (Section of Physical Education, Anhui Medical University, Hefei 230032) Abstract: In order to investigate the mechanism of myocardial apoptosis by exercise, we studied the effects of different exercise intensities on apoptotic gene Bcl-2, Bax and Caspase-3 in rat myocardium cells. The rats were randomly divided into three groups (control group, aerobic exercise group and overtraining group), the mRNA and protein expressions of apoptotic gene Bax, Bcl-2 and Caspase-3 were observed in myocardium cells by quantitative fluorescent polymerase chain reaction (qPCR) and westernblot. As results, compared with the control group, the mRNA and protein expressions of Bcl-2 both increased in aerobic exercise group which inhib- ited myocardial apoptosis. However, the decreased Bcl-2 expression and the increased expressions of Bax