赤眼鳟TRAF6基因的cDNA克隆及其.PDFVIP

() 2016, 42(6):641–646. DOI:10.13331/ki.jhau.2016.06.011 Journal of Hunan Agricultural University (Natural Sciences) 赤眼鳟 TRAF6 基因的 cDNA 克隆及其 应对 GCRV 的免疫表达特性 1,2 1# 1,2 1 1 1 1,2* (1. 410128 2. 415000) 摘 要(Squaliobarbus curriculus)6 GCRV RACE TRAF6 cDNA (2 621 bp)5′ 50 bp1 629 bp3′942 bp 542 61 6706.01SMART TRAF6 1 RING 2 1 1 MATH TRAF6 TRAF6 PCR TRAF6 12 GCRV TRAF6 24 h TRAF6 GCRV 关 键 词6 (GCRV) 中图分类号Q785; S965 文献标志码A 文章编号10071032(2016)06064106 Molecular cloning and expression characteristics response to GCRV of TRAF6 in Squaliobarbus curriculus 1,2 1# 1,2 1 1 1 1,2* Chen Kaijian , Wang Jing′an , Liu Qiaolin , Wang Ronghua , Xu Ying , Zhao Xin , Xiao Tiaoyi (1.Hunan Engineering Research Center for Utilization of Characteristics of Aquatic Resources, Hunan Agricultural University, Changsha 410128, China; 2.Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, Hunan 415000, China) Abstract: In order to investigate whether tumor necrosis factor-associated factor 6 gene involved inantiviral immune response, the full-length cDNA of TRAF6 in Squaliobarbus curriculus was cloned by using RACE–PCRs method. The full-length cDNA of TRAF6 is 2 621 bp including a 5′–terminal untranslated region of 50 bp, a 3′–terminal untranslated region of 942 bp and an open reading frame of 1 629 bp encoding a polypeptide of 542 amino acid residues. The putative isoelectric point and molecular weight of TRAF6 was 6.01 and 61 670, respectively. The protein encoded by this gene includes one ring