甲壳素生产废水提取虾青素及水解蛋白工艺研究.pdfVIP

《 饲料工业》· 年第 卷第 期 试验研究 2011 32 14 甲壳素生产废水提取虾青素及水解蛋白的工艺研究 刘汉文 王爱民 陈洪兴 张其林 摘 要：以小龙虾壳为原料，按酸碱交替法生产的甲壳素，蛋白质和虾青素等物质流失于废水中， 采用壳聚糖作絮凝剂，可将虾青素与蛋白质絮凝沉淀。试验结果表明：调节pH 值6.0 ，每升废水中添加 40 ml 1%壳聚糖醋酸水溶液，静置3 h，废水中溶液的透光率最低，絮凝物蛋白质回收率达88.6%。使 用几种蛋白酶水解絮凝物，其中风味蛋白酶水解度最大，在初始pH 值6.5 ，水解温度55 ℃,试验确定： 选用风味蛋白酶1.5 g/kg 絮凝物，固液比1 ： 2 时，保温酶解时间4 h ，虾青素提取率可达4.49% 。该条 件下絮凝物蛋白质的水解度为23.8% 。提取虾青素并将水解后的蛋白浓缩，喷雾干燥，制成水解动物 蛋白，产品不苦，具有良好的风味。 关键词：小龙虾壳；壳聚糖；絮凝；酶解；虾青素；水解蛋白；水解度 中图分类号： 文献标识码： 文章编号 S963.3 A ：1001-991X （2011）14-0051-05 Research on the extraction of astaxanthin and HAP in wastewater after crayfish shell processing Liu Hanwen, Wang Aimin, Chen Hongxing, Zhang Qilin ： Abstract The shortcoming for the acid-base alternative to produce chitin from the crayfish shell was that the protein and astaxanthin were lost in the wastewater. In the current study, the chitosan was used as flocculant and the astaxanthin and protein was coagulated and sedimentated easier. The results showed that the OD reached lowest and the recycled rate reached the maximum at 88.6% when the 40 ml 1% chitosan acetic acid solution was added into the solution (pH 6.0) and stood for 3 h. The enzyme activity of several enzymatic hydrolysis floc were compared and the results revealed that the flavourzyme DH was highest at pH 6.5 and 55 ℃ . Those results determined the optimal possessing method was: adding flavourzyme 1.5 g/kg, S/L 1 ： 2, and hydrolyzing for 4 h, and the extraction rate of astaxanthin could reach to 4.49%. Under those c