水酶法提取紫苏籽油脂和蛋白质的工艺条件_徐冰冰.pdf

2012, Vol.33, No.22 127 1,2 1,* 1 1 2 (1. 1000372. 471003) pH Bo x-Behnken 1:4(g/mL)pH8.9461.3 ()1.5%4.47h 85.59%73.43% Optimization of Aqueous Enzymatic Extraction of Oil and Protein from Perilla Seeds 1,2 1,* 1 1 2 XU Bing-bing WANG Ying-yao LUAN Xia DUAN Zhang-qun LIU Jian-xue (1. Academy of State Administration of Grain, Beijing 100037, China 2. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China) Abstract Response surface methodology was employed to optimize the aqueous enzymatic extraction of oil and protein from perilla seeds. The extraction yields of protein and oil were investigated as a function of six extraction conditions including enzyme type, solid-to-solvent ratio, pH, temperature, enzyme loading and hydrolysis time. As a result, a mathematic model was established using a Box-Behnken experimental design. The model was statistically signifi cant and allowed accurate prediction of extraction yields of protein and oil under various conditions. Alcalase was identifi ed as the best enzyme for the aqueous enzymatic extraction of oil and protein from perilla seeds and the optimized values of solid-to-solvent ratio, pH, temperature, enzyme loading, and hydrolysis time for extracting oil and protein from perilla seeds were 1:4, 8.94, 61.3 , 1.5% and 4.47 h, respectively. Under the optimized conditions, the extraction yields of oil and protein were e 85.59% and 73.43%, respectively. Key words perilla seed aqueous enzymatic extraction optimization response surface analysis oil extraction yield