菊芋块茎多酚氧化酶特性.pdf

20 10, 19( 8) : 185187, 206 A cta A g r ic ult ur ae B or ealiocc idental is S in ica 李璟琦 ( , 710061) : , ( P PO) , PP O pH P PO , pH 7. 4, pH 3. 5 ; 45 , 70 , , 80 , , 80 2 min ; 0. 04 mol ! L- 1 P PO : ; ; ; : T S201. 1 : A : 20 10) 080 18503 Polyphenol Oxidase Characteristics of Jerusalem Artichoke LI ingqi ( College of Lif e Science, Shaan xi Institute of Education , Xi∀ an 710061, China) Abstract: T he activ ities of polyphenol ox dase ( PPO) w ere studied in this paper by spectrophoto metry including conditions of optimum pH , optimum temperatur e, stability to heat, and the effect of diff er ent inhibitors. T he results indicated that the optim um pH and temperature of artichoke PP O were 7. 4 and 45 respectively. T he enzy me activ ity w as suppressed by 80 heating treatment for 2 m in. Cit r ic acid ( CA) and NaH SO3 w ere strong inhibitors, the effective concentr ation of these inhibitor s w as 0. 04 mol !L- 1 . Key words: Artichoke; Poly phenol ox idase ( PPO) ; Enzym e activity; Brow ning [ 6] , , , , PPO [ 710] , , PPO [ 1112] , , 79. 8%16. 6% 1% PP O , , 16. 6%2. 8% , , 78% , 1 , 1. 1 , , [ 13] , , [ 45] , ,