细胞生物名词解释(国外英文资料).docVIP

细胞生物名词解释(国外英文资料) 1 cell culture (cell culture) : cells in vitro culture technique, namely the aseptic condition, removed from the body tissues or cells, the normal physiological state simulator under the basic condition of survival, let it continue to exist in a petri dish, growth and reproduction. Primary culture (primary culture) : directly derived from the cell culture of organism tissue. 3 transfer culture (secondary culture) : the growth of the cultured cells from the cultured bottles, and the process of the cultivation of a ratio of 1:2 or more. Four Northern blot: the RNA extracted from the organization of mixture with agarose gel electrophoresis separation by size, by imprinting method after separation of nucleic acid is transferred to nitrocellulose membrane or nylon membrane, again with a specific DNA probe hybridization, testing complementary RNA molecules, a process called Northern blot. Five Southern blot: DNA extracted from the organization of mixture with agarose gel electrophoresis separation by size, by imprinting method after separation of nucleic acid is transferred to nitrocellulose membrane or nylon membrane, again with a specific DNA probe hybridization, testing complementary DNA molecules, a process called Southern blot. Six Western blot: protein extracted from tissues or cells of mixture with polyacrylamide gel electrophoresis separation by size, reoccupy after imprinting method to separate the protein bands of the same transferred to nitrocellulose membrane or nylon membrane, with a specific antibody for hybridization, detection of protein molecules, a process called Western blot. Seven active transport (active transport), mediated by carrier protein substances or electrochemical gradient, against a concentration gradient by the side of the low concentration to high concentration on one side of the way for transport across the membrane. 8 passive transport (passive transport) : by a carrier protein or channel proteins mediated material suit