综合试验申报 邻二氮菲分光光度法测定微量铁(国外英文资料).docVIP

综合实验报告 邻二氮菲分光光度法测定微量铁(国外英文资料) O-diazo spectrophotometry determination of trace iron One, the purpose of the experiment The method of determining the experimental conditions, and the method of measuring the trace iron by means of the diazo spectrophotometry; The method of using 721 spectrophotometer is used, and the main structure of this instrument is understood. Second, the experimental principle Manner suitable conditions were determined: in visible spectrophotometry determination of analyte is usually will react with chromogenic agent, make the generation of non-ferrous material, then the absorbance measurement, then the content of the material being tested. Therefore, the complete degree of color condition and the measurement condition of absorbance will affect the accuracy of the measurement. In order to make the measurement have higher sensitivity and accuracy, it is necessary to select the suitable color response condition and instrument measurement condition. Usually studied chromogenic reaction conditions are color temperature and time, chromogenic agent, chromogenic solution acidity, the influence factors of interfering substance and eliminate, but mainly measuring wavelength and the choice of the reference solution. The choice of the dosage of the colorant and the measurement wavelength is the content of the experiment. 2. How to determine the appropriate conditions: test the condition of general steps to change one of the factors, temporary fixed and the other factors, after the color measuring absorbance corresponding solutions, through the absorbance and the curve of change factors to determine the appropriate conditions. The principle of iron content in industrial hydrochloric acid was determined by this experiment: according to the lambert beaears law: A = epsilon BC. The absorbance A of colored material is directly proportional to the concentration of the substance in A certain concentration range when the incident wavelength of light and the light