水通道蛋白1在鼻息肉中的表达及其调节机制.docVIP

水通道蛋白1在鼻息肉中的表达及其调节机制 【摘要】 研究 水通道蛋白1(aquaporin1, AQP1)在鼻息肉组织中的表达,探讨其与鼻息肉水肿形成及 发展 的关系。 方法 ：取鼻中隔偏曲矫正术患者下鼻甲黏膜30例和鼻息肉组织60例。鼻息肉组型1期10例, 2期15例, 3期25例，型10例。采用免疫组织化学检测AQP1在不同类型及不同临床分期鼻息肉和下鼻甲黏膜组织中的表达及在不同类型鼻息肉组织中的表达。结果：（1）鼻息肉上皮层AQP1阳性细胞数显著高于下鼻甲黏膜(Ｐ＜0 01); 不同分型分期鼻息肉上皮层AQP1的阳性细胞数又有差异,型3期和型鼻息肉显著低于型1期鼻息肉(Ｐ＜0 01); 而在鼻息肉血管内皮及腺体细胞中的AQP1的表达明显高于下鼻甲(Ｐ＜0 01); （2）型3期和型鼻息肉血管内皮中AQP1阳性细胞数显著高于型1期鼻息肉和下鼻甲黏膜上皮下组织(Ｐ＜0 01)。结论：AQP1在鼻息肉组织中的异常表达可能与鼻息肉的发生和发展密切相关, 具体机制有待进一步研究。 【关键词】 水通道蛋白1 鼻息肉 免疫组织化学 ［ABSTRACT］ Objective: To explore the expression and distribution of aquaporin1(AQP1) in the formation and development of nasal polyps. Methods: Thirty cases of normal inferior turbinates and sixty cases of nasal polyps were enrolled in this study. In the nasal polyp group, there were 10 cases of type II phase 1, 15 cases of type II phase 2, 25 cases of type II phase 3, and 10 cases of type III. Expression of AQP1 in both groups was determined by the Elivision plus immunohistochemistry technique. Results: (1) Positive AQP1 cells in the epithelial cell layer of nasal polyps were significantly fewer than those of the inferior turbinate (P＜0.01) and positive AQP1 cells of nasal polyps of the type Ⅱ phase 1 were significantly more than those of the type Ⅲ or type Ⅱ phase 3 (P＜0.01). (2) Positive AQP1 cells in both the vessel endothelium and the glandular epithelium from the type , type Ⅱ phase 3 of nasal polyps were significantly more than those from the type Ⅱ phase 1 nasal polyps and the inferior turbinate (P＜0.01). Conclusion: AQP1 might play an important role in the formation and development of nasal polyps. Exploration of the regulation of AQP1 contributes toward evaluating the pathogenesis of nasal polyps. ［KEY WORDS］ Aquaporin1; Nasal polyps; Immunohistochemisty 鼻息肉是耳鼻喉科常见病，发病率较高，复发性强［1］。临床上最常见的病理类型是水肿型(约占总数的85% 90%)，其具体发生机制尚不明确［2］。现认为鼻息肉的发病是多因素、多步骤的过程，涉及众多复杂的病理机制。 AQP1是上世纪80年代末由Agre发现的第一个水通道，被称为原型水通道。AQP1在人体内分布广泛，存在于不同的组织器官中，如与液体被吸收和分泌有关的肾小管、髓袢降支薄壁段、脉络膜丛、唾液腺上皮、支气管、肺泡上皮、毛细血管内皮等。在淋巴管和黏膜下毛细血管中的AQP1能促