瓣膜病慢性心房颤动心房肌缝隙连接CX43与CX40的重构.pdf

2004 8 84 Chin J CardiacArrhyth, 2004, Vol. 8 No.4 223 CX43 CX40 贾玉和 张澍 王方正 浦介麟 马坚 ! ( ) Connexin 43 ( CX43) Connexin 40 (CX40) ( 1) 11 ( ) ( 8 , 3 ) , Northern blot CX43 CX40 m NA ; ( 2) 20 ( 8 , 12 ,3 15 ) , ( confocal laser scanning mi croscopy) CX43 CX40 ( 1) CX43 CX40 m NA ; (2)CX43 CX40 , CX40 ( P 005) , CX43 CX40 , CX43 CX40 m NA , CX40 , , , CX40 , ! ; ; ; Connexin43; Connexin40; Remodeling of gapjunctional proteins, connexin40 andconnexin43, in human atrial myocardium of valvu lar permanent atrial fibrillation JIA Yuhe, ZHANG Shu, WANG Fangzheng, PUJielin, MA Jian Center of Arrhythmia iagnosis and Treatment, Fuwai Hospital, CAMS and PUMC, Beij ing 100037, China Abstract! Objective To reveal the changes of connexin40 ( CX40) and connexin43 ( CX43) m NA level, spatial distribution pattern, and their morphology in human atrialmyocardium of permanent atrial fibril lation and sinus rhythm in valvular heart disease Methods Eleven cases of human right atrial appendage ( AA) samples were collected from rheumatic heart disease during valve replacement, eight of which were a trial fibrillation (AF) samples that average sustained AF period were ( 56∀ 65) years, three of which re mained sinus rhythm ( S ) Total NA was purified, CX40 and CX43 m NA level were analysised through Northern blot technique Twenty cases of human AA samples from patients with rheumatic heart disease were collected (eight S and twelve AF) Frozen sections ( 8 m) were cut from longitudinal direction and transverse direction of myocardium respectivelyAll sections were incubated with antiCX43 and CX40 anti body, and with appropriate secondary antibody (IgG) , and then immunolabeled sections were examined using a confocal microscopy The fluorescent plaque area and spatial distribution pattern and morphology of CX40 and CX43