山豆根对实热证小鼠抗炎作用效―毒―证关联评价.docVIP

山豆根对实热证小鼠抗炎作用的效―毒―证关联评价 　　[摘要]建立小鼠咽喉实热证模型，给予山豆根水提组分，检测血中热证指标和肝、肾功能指标的变化。通过考察并比较山豆根水提组分对小鼠发挥抗炎作用剂量下的伴随毒副作用及其机制进行研究，阐释有毒中药山豆根“功效-毒性-证候”相关性。结果山豆根水提组分对混合致炎液所致咽喉实热证模型小鼠的耳肿胀具有较强的抑制作用，连续多次给药可引起小鼠血中ALT，AST活性明显升高，肝脏系数增大，血中PGE2，SOD，MDA，NO，NOS，GSH，GSH-Px等指标有明显改变。表明，山豆根水提组分对混合致炎液所致咽喉实热证模型小鼠耳肿胀具有较强的抑制作用，且比对正常动物强，药效剂量下的山豆根还可产生一定的肝脏毒副作用。山豆根对咽喉实热证小鼠的抗炎作用及肝毒性机制均与过氧化损伤有关 [关键词]山豆根;水提组分;咽喉实热证;抗炎;毒副作用 Assessment of efficacy-toxicity-syndrome correlation based on anti-inflammation of Sophorae Tonkinensis Radix et Rhizoma in excess-heat mice SUN Rong1*， FENG Qun2， XIE Yuan-zhang3， YANG Qian4， LI Su-jun5 （1. Shandong Academy of Chinese Medicine， Ji′nan 250014， China; 2. Tianjin University of Traditional Chinese Medicine， Tianjin 300193， China; 3. Yantai Rongchang Pharmaceutical Co. Ltd.， Yantai 264006， China; 4. Rizhao Hospital of TCM， Rizhao 276800， China; 5. Shandong Food and Drug Administration， Ji′nan 250013， China） [Abstract]Models of throat excess-heat mice were established and different dosages of water extract of STRR were ig given to mice to observe anti-inflammatory effect and its mechanism. The activities of ALT， AST and the contents of TNF-α， T3， rT3， T4， SOD， MDA， PEG2， NO， NOS， Cr， BUN， GSH and GSH-Px in serum were tested while liver index， kidney index， spleen index and thymus index were measured. The anti-inflammatory efficacy accompanied by side effects and mechanisms of water extract of Sophorae Tonkinensis Radix et Rhizoma （STRR） in excess-heat mice were investigated to clear safety dose-dependence range and the relationship of efficacy， toxicity and syndrome. In the experiment， water extract of STRR showed a strong inhibitory effect on ear edema by croton oil in throat excess-heat mice. The activities of ALT， AST in serum and liver index were all higher than that of normal group after multiple administration. PEG2， SOD， MDA， NO， NOS， GSH and GSH-Px had obvious changes. According to the results， water extract of STRR has an anti-inflammatory eff