水酶法提取板栗壳棕色素工艺条件的筛选.PDFVIP

第33 卷 第2 期 经 济 林 研 究 Vol. 33 No.2 2015 年6 月 Nonwood Forest Research Jun. 2015 Doi:10.14067/j .cnki.1003-8981.2015.02.018 http : // 水酶法提取板栗壳棕色素工艺条件的筛选 许 伟，姜慧仙，郑 剑，仲山民，王 超，常银子 （浙江农林大学 农业与食品科学学院，浙江 临安 311300 ） 摘 要：为了确定水酶法提取板栗壳棕色素的工艺条件，以板栗壳为原料，通过单因素试验和正交试验，对其 工艺条件进行筛选。结果表明，水酶法提取板栗壳棕色素的最适工艺条件为：复合酶总酶量2.0%，纤维素酶、 中性蛋白酶、果胶酶质量比为2 ∶3 ∶5，酶处理pH 值5.0，温度50 ℃，时间2.0 h ，料液比1 ∶120 。 关键词：板栗壳；棕色素；复合酶；工艺条件 中图分类号：S664.2 文献标志码：A 文章编号：1003—8981(2015)02—0101—06 Screening of extraction process conditions of brown pigment from chestnut shell by enzymatic hydrolysis method XU Wei, JIANG Hui-xian, ZHENG Jian, ZHONG Shan-min, WANG Chao, CHANG Yin-zi (School of Agriculture and Food Science, Zhej iang Agriculture and Forestry University, Lin an 311300, Zhej iang, China) ’ Abstract: In order to obtain the optimal extraction process conditions of brown pigment from chestnut shell by enzymatic hydrolysis method, taking chestnut shell as materials, the process conditions were screened by several single factor experiments and a orthogonal experiment. The results showed that the optimal extraction process conditions were 2.0% composite enzymes, the mass ratio 2 ∶3 ∶5 of cellulose, neutral protease and pectinase, reaction pH value 5.0, temperature 50 ℃, duration 2.0 h, and solid-liquid ratio 1 ∶120. Key words: chestnut shell; brown pigment; composite enzymes; process conditions 板栗Castanea mllissima Blume 属壳斗科Fagaccac 粉等被分解，使细胞内色素成分充分释放出来， 栗属坚果类植物，为多年生落叶乔木。板栗在我国 同时具有工艺简单，条件温和，对活性成分破坏小， 分布多达26 个省（市、自治区），产量