基因图谱的描述.docVIP

进化关系的描述 As shown in Fig. 5,HbzF is located in an unrelated branch with maleylpyruvate isomerases and most closely related to the hydrolases for different substrates. *The L-cysteine-dependent maleylpyruvate isomerase represented by BagL forms an independent clade with a closer relationship to BSHS-transferases or MSH-dependent maleylpyruvate isomerase than to MSH S-transferases. （系统发生关系远近）差异的表达 *From an evolutionary perspective, these ?ndings indicate that the reductase gene npcB has a relationship to the tcp and had genes that is more distant than the evolutionary relationship of the corresponding monooxygenase genes (npcA, tcpA, and hadA) and the corresponding hydroxyquinol 1,2-dioxygenase genes (npcC, tcpC, and hadC). Iso of P. putida S16 is in a deep branch of its own. Within the alpha/beta-hydrolase family, BLAST analysis indicated that the ORF243 protein showed similarity to a group of proteins that includes Pseudomonas putida DmpD, TodF, and XylF, Pseudomonas azelaica HbpD, Rhodococcus sp. EtbD and BphD, and P. ?uorescens CumD. All these proteins are hydrolases that are involved in the degradation of various aromatic compounds including xylene and toluene. A phylogenetic analysis showed ORF243 to be the most distantly related within this group of proteins, being somewhat more closely related to HbpD and BphD, to which it shows 12% identity and 20% similarity, respectively.（也就是说ORF 243 与 这个水解酶家族中的蛋白相似性很低，相比较而言与HbpD and BphD 的相似性高点，分别达到12% 和 20%） 出自： 基因/蛋白位置关系的表达方法 *This 558-amino-acid protein has a salicylate 1-monooxygenase domain located between positions 1 and 435 in the N terminus and a cytochrome b5-like heme binding domain between positions 483 and 555 in the C terminus (Fig. 1A). *One GlxR-binding site (DFMx) was found to be located -13 to +8 bp upstream of the genDFM promoter. *The GenR binding site R-KHn01 (located between positions -47 and -16) overlapped the -35 region of the genKH promoter sequence and is involved in positive regulation of its tran