基干α―葡萄糖苷酶抑制活性评价桑叶多组分药效相互作用探讨.docVIP

基于α―葡萄糖苷酶抑制活性评价桑叶多组分药效相互作用研究 　　[摘要] 采用柱色谱法对桑叶提取物中黄酮、生物碱、多糖组分进行富集分离；采用葡萄糖氧化酶法，利用蔗糖为底物，以α-葡萄糖苷酶抑制模型对桑叶多组分进行评价；采用等效线法、周-特氏联合指数法和等辐射分析法评价2组分间的药效相互作用及量效特点，为揭示桑叶降血糖的作用机制提供科学依据。成分分析表明，桑叶提取物中，黄酮质量分数为5.3%，有机酚酸质量分数为10.8%，1-脱氧野尻霉素（DNJ）质量分数为39.4%，多糖质量分数为18.9%。活性评价表明，桑叶黄酮、生物碱、多糖组分均具有显著的α-葡萄糖苷酶抑制活性，其抑制率随各组分浓度的增加而升高，3个组分中桑叶生物碱抑制活性最强。桑叶生物碱与桑叶黄酮配伍、桑叶生物碱与桑叶多糖配伍均表现出协同增效作用，但多糖与黄酮组配伍未见明显的协同作用，从而证实了桑叶多组分调节血糖的相互作用，为桑叶多组分降血糖物质基础与作用机制的揭示提供了科学依据 [关键词]桑叶； α-葡萄糖苷酶； 相互作用； 协同增效； 量效关系 [Abstract]Column chromatography was used for enrichment and separation of flavonoids， alkaloids and polysaccharides from the extracts of Morus alba leaves； glucose oxidase method was used with sucrose as the substrate to evaluate the multi-components of M. alba leaves in α-glucosidase inhibitory models； isobole method， Chou-Talalay combination index analysis and isobolographic analysis were used to evaluate the interaction effects and dose-effect characteristics of two components， providing scientific basis for revealing the hpyerglycemic mechanism of M. alba leaves. The components analysis showed that flavonoid content was 5.3%； organic phenolic acids content was 10.8%； DNJ content was 39.4%； and polysaccharide content was 18.9%. Activity evaluation results demonstrated that flavonoids， alkaloids and polysaccharides of M. alba leaves had significant inhibitory effects on α-glucosidase， and the inhibitory rate was increased with the increasing concentration. Alkaloids showed most significant inhibitory effects among these three components. Both compatibility of alkaloids and flavonoids， and the compatibility of alkaloids and polysaccharides demonstrated synergistic effects， but the compatibility of flavonoids and polysaccharides showed no obvious synergistic effects. The results have confirmed the interaction of multi-components from M. alba leaves to regulate blood sugar， and provided scientific basis for revealing hpyerglycemic effectiveness and mechanism of the multi-components from M. alba leaves. [Key w