2003 西班牙 阿威链霉菌转化啤酒糟为羟基苯乙烯酸.pdf

Enzyme and Microbial Technology 32 (2003) 140–144 Growth and release of hydroxycinnamic acids from Brewer’s spent grain by Streptomyces avermitilis CECT 3339 Begoña Bartolomé a , Carmen Gómez-Cordovés a a ´ b , Ana I. Sancho , Nardy Dıez , Patricia Ferreira b , Juan Soliverib , José L. Copa-Patiñob,∗ a Department of Sectorial Technologies, Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain b Department of Microbiology and Parasitology, University of Alcalá, Alcalá de Henares, 28871 Madrid, Spain Received 3 January 2002; received in revised form 3 October 2002; accepted 5 October 2002 Abstract Streptomyces avermitilis CECT 3339 is grown on Brewer’s spent grain (BSG) and the production of feruloyl esterase (FAE) and (1 → 4)--d-xylan xilanohydrolase (xylanase) activities is studied over 5 days. Maximum level of xylanases was found at day 1. FAE activity on methyl ferulate reached a maximum level at day 2, whereas FAE activity on feruloylated oligosaccharides, either from wheat bran or sugar-beet pulp, was maximal at day 1. The cultures (1–5 days) from S. avermilitis CECT 3339 grown on BSG and on other two agro-industrial residues such as de-starched wheat bran and sugar-beet pulp were tested for the release of hydroxycinnamic acids (ferulic and p -coumaric acids) from BSG. Most ferulic acid (FA) was released when culture supernatants from day 1 and BSG as carbon source (43% of total alkali-extractable ferulic acid) and from day 2 and de-starched wheat bran as carbon source (41.2%) were used. The level of p -coumaric