亚油酸通过调控胰岛素信号通路抑制胰岛素抵抗.pdf

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2015, Article ID 643102, 9 pages /10.1155/2015/643102 Research Article Oleanolic Acid Attenuates Insulin Resistance via NF-砑匓 to Regulate the IRS1-GLUT4 Pathway in HepG2 Cells Ming Li, Zongyu Han, Weijian Bei, Xianglu Rong, Jiao Guo, and Xuguang Hu Key Unit of Modulating Liver to Treat Hyperlipemia SATCM (State Administration of Traditional Chinese Medicine), Level 3 Lab of Lipid Metabolism SATCM, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China Correspondence should be addressed to Jiao Guo; gyguoyz@163.com and Xuguang Hu; hxguang21@163.com Received 10 October 2015; Revised 9 December 2015; Accepted 15 December 2015 Academic Editor: Yuewen Gong Copyright 漏 2015 Ming Li et al. his is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. he aim of our study is to elucidate the mechanisms of oleanolic acid (OA) on insulin resistance (IR) in HepG2 cells. HepG2 cells were induced with FFA as the insulin resistance model and were treated with OA. hen the glucose content and the levels of tumor necrosis factor-砘 (TNF-砘? and interleukin-6 (IL-6) were analyzed. Moreover, protein expression of nuclear factor kappa B (NF- 砑匓), insulin receptor substrate 1(IRS1), and glucose transporter 4 (GLUT4) in cells treated with OA were measured by Western blot analysis. Additionally, IRS1 protein expression exposed to OA was detected ater using pyrrolidine dithiocarbamate (PDTC).Our results revealed that OA decreased the glucose content in HepG2 cells in vitro