48-基因治疗.pdf

Biomaterials 32 (2011) 4849e4856 Contents lists available at ScienceDirect Biomaterials journal homepage: www.elsevi /locate/biomaterials A cationic prodrug/therapeutic gene nanocomplex for the synergistic treatment of tumors Xiao Lu a, b, Qing-Qing Wang c, Fu-Jian Xu b, *, Gu-Ping Tang a, **, Wan-Tai Yang b a Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China b Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science Engineering, Beijing University of Chemical Technology, Beijing 100029, China c Institute of Immunology, Zhejiang University, Hangzhou 310058, PR China a r t i c l e i n f o a b s t r a c t Article history: The combination of gene therapy and chemotherapy may increase the therapeutic efficacy in the treatment of Received 6 January 2011 patients. In this work, the cationic polymer prodrug/plasmid nanocomplexes were designed to in vivo Accepted 9 March 2011 synergistically treat drug-resistant breast tumors. Cationic b-cyclodextrin-polyethylenimine-Dox (PC-Dox) Available online 31 March 2011 conjugates were prepared for carrying wt p53 plasmid in the form of PC-Dox/p53 nanocomplexes to achieve synergistic cancer therapeutic effects of drug and gene therapies. Such PC-Dox/p53 nanocomplexes ensure Keywords: that both drug and gene can be delivered to the same cancer cells. The physicochemical properties and Dox DNA