PBA 测定大鼠尿液中糖皮质激素.pdfVIP

Journal of Pharmaceutical and Biomedical Analysis 107 (2015) 450–455 Contents lists available at ScienceDirect Journal of Pharmaceutical and Biomedical Analysis j o u r n a l h o me p a ge : w w w. elsev /lo ca t e /j p b a A simple LC–MS/MS method for the determination of cortisol, cortisone and tetrahydro-metabolites in human urine: Assay development, validation and application in depression patients Xuejia Zhai, Fen Chen, Chaoran Zhu, Yongning Lu ∗ Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China a r t i c l e i n f o a b s t r a c t Article history: Chronic stress as well as major depressive disorders is associated with cortisol metabolism. Two enzymes Received 24 October 2014 modulate cortisol (F) and cortisone (E) interconversion: 11-hydroxysteroid dehydrogenase type 1 and Received in revised form 16 January 2015 type 2 (11-HSD1 and 11-HSD2 ). Furthermore, F and E were inactivated by 5 and 5 reductases to their Accepted 19 January 2015 tetrahydro-metabolites: tetrahydrocortisol (THF), allo-tetrahydrocortisol (5-THF) and tetrahydrocorti- Available online 25 January 2015 sone (THE). To better understand depression a LC-MS/MS method for simultaneous determination of F, E THF, 5-THF and THE in human urine has been developed and validated. The quantification range was Keywords: −1