高糖对小鼠骨髓间充质干细胞增殖影响.docVIP

高糖对小鼠骨髓间充质干细胞增殖的影响 　　摘 要：采用不同浓度葡萄糖对间充质干细胞（MSCs）增殖的影响来研究运动员补糖的MSCs效应，分别用5.0和22.5 mmol/L葡萄糖预处理小鼠MSCs细胞株C3H10T1/2 48 h后，换不同浓度葡萄糖（5.0～500.0 mmol/L）持续培养或50.0～150.0 mmol/L和22.5 mmol/L进行12 h交替培养，甲基噻唑基四唑（MTT）法检测24～192 h的细胞增殖活性。结果发现：1）22.5和50.0 mmol/L葡萄糖组细胞的增殖活性最佳，并称为正糖。浓度低于或高于正糖的葡萄糖称为低糖或高糖。2）22.5 mmol/L正糖预处理后换不同糖浓度持续培养时，和正糖相比，高糖100.0～300.0 mmol/L具有短暂促增殖作用。3）高糖长期培养抑制细胞增殖。结果说明：高糖对正糖预处理的MSCs具有短暂促增殖效应 关 键 词：运动生物化学；间充质干细胞；正糖；高糖；小鼠 中图分类号：G804.7 文献标志码：A 文章编号：1006-7116（2015）06-0139-06 Abstract： In order to study the mesenchymal stem cells （MSCs） effect of carbohydrate supplementation for athletes by studying the effect of glucose in different concentrations on the proliferation of MSCs， the authors pretreated cell lines C3H10T1/2 of MSCs of mice with 5.0 and 22.5 mmol/L glucose respectively for 48 h， then continuously cultured them with glucose in different concentrations （5.0～500.0 mmol/L） or alternatively cultured them with 50.0～150.0 mmol/L and 22.5 mmol/L glucose for 12 h， measured the proliferation activity of 24～192 h cells by means of methylthiazolyldiphenyl-tetrazolium bromide/thiazolyl blue tetrazolium bromide （MTT） assay， and revealed the following findings： 1）the proliferation activity of the cells cultured with 22.5 and 50.0 mmol/L glucose was the best， and the glucose was called as normal glucose， while the glucose whose concentration was lower or higher than normal glucose was called as low glucose or high glucose； 2）when MSCs were pretreated with 22.5 mmol/L normal glucose and then continuously cultured with glucose in different concentrations， as compared with normal glucose， 100.0～300.0 mmol/L high glucose had a short-term proliferation promoting function； 3）longer-term high glucose culturing restrained cell proliferation. The said findings indicate that high glucose has a short-term proliferation promoting effect on MSCs pretreated with normal glucose. Key words： sports biochemistry；mesenchymal stem cell；normal glucose；high glucose；mouse 间充质干细胞（mesen